中文摘要：

目的：检测质膜微囊结构蛋白caveolin-2在内皮-单核细胞激活多肽-II（EMAP—II）增强血肿瘤屏障（BTB）通透性过程中的表达水平变化。方法：荷瘤Wistar大鼠被随机分成4组（每组16只）：EMAP—II处理0h、1h、2h和4h组。采用伊文思蓝渗透性实验评估各组BTB通透性变化情况；RT—PCR、Westernblot．ting、免疫组化和免疫荧光法检测脑胶质瘤组织毛细血管上caveolin-2的表达水平变化。结果：EMAP—II作用1h时，BTB的通透性显著增强，随后逐渐降低，4h时恢复正常；同时，EMAP—II作用1h时，脑胶质瘤组织毛细血管上caveolin-2的表达水平显著增高，随后逐渐降低，4h时恢复正常。结论：EMAP—II可能通过cayeolae介导的跨细胞途径来增强BTB的通透性，其机制与caveolin-2的表达水平上调有关。

英文摘要：

Objective： To detect changes in the expression level of caveolar structural protein caveolin - 2 in endo- thelial monocyte activating polypeptide- II（ EMAP -II ） -induced increase in blood -tumor barrier（ BTB ）permea- bility. Methods：Tumor- bearing rats were randomly divided into 4 groups（ n = 16, each） ： EMAP- II Oh, l h ,2h and 4h groups. Evans blue method was used to evaluate the alterations in BTB permeability. Changes in the expression lev- el of the caveolar structural protein caveolin - 2 in brain capillaries were measured by RT - PCR, Western blotting, immunohistochemistry and immunofluorescent assays. Results：The EB content in tumor tissues increased significantly at 1 h after EMAP - II administration, and trended back toward the level of the EMAP - II Oh group gradually by 4h. The expression levels of caveolin -2 in brain capillaries were significantly increased at lh, and decreased gradually toward the untreated level thereafter. Conclusion： EMAP - II may induce BTB hyperpermeability via caveolae - medi- ated transcellular pathway, which is associated with the upregulation of caveolin - 2.