中文摘要：

有机染料和量子点等荧光标记材料存在发射光谱宽、光热稳定性差和细胞毒性等缺陷,限制了其在生物学研究中的应用.镧系掺杂上转换发光材料不存在＂自发荧光＂和＂光漂白＂现象,灵敏度高,长期稳定性好,利于活体检测.论文首次采用热解法,以油酸和十八烯为表面活性剂和溶剂,制备了KY3F（10）：Yb,RE（RE=Er,Ho,Tm）纳米晶.研究了油酸含量对产物形貌和粒径尺寸的影响,当油酸与十八烯的比例为3：1时,为制备类球形单分散纳米晶体的最佳工艺条件,在980 nm半导体激光器激发下,样品KY3F（10）：Yb,RE（RE=Er,Ho,Tm）分别发射出较强的黄绿、绿色和蓝色光,这些结果显示KY3F（10）：Yb,RE（RE=Er,Ho,Tm）纳米粒子作为生物探针在多重荧光标记方面具有优异的特性.

英文摘要：

Organic dyes and quantum dots possess the defects： wide emission spectrum and the poor photothermal stability and cytotoxicity, which restricts their applications in biological studies. Lanthanide-doped upconversion fluorescent materials in which there exists neither ＂autofluorescence＂ nor ＂light bleaching＂ phenomenon, has high sensitivity and good long-term stability, which are conducive to in vivo detection. KY3F10： Yb, RE（RE = Er, Ho, Tin） nanocrystals are synthesized by the thermal decomposition method and oleic acid as surface coating agent. The effects of the oleic acid content on morphology and particle size are studied. Optimal ratio of oleic acid to octadecene is 3：1 for the preparation of spherical monodisperse nanocrystals. Strong yellow green, green and blue emissions from the prepared samples are observed at 980 nrn laser excitation. These results show that KY3F10： Yb, RE （RE= Er, Ho, Tin） nanoparticles as biological probes have excellent features in multiple fluorescent markers.