中文摘要：

用胶质芽孢杆菌作吸附剂，探讨了采用生物吸附-浮选法去除水相中Pb^2＋的可能性，并对生物吸附机理和吸附剂与捕收剂的作用机理进行了分析。结果表明，用阳离子型捕收剂对胶质芽孢杆菌有较高的去除率，在pH为3-6的范围内，用十二胺浮选吸附了Pb“的胶质芽孢杆菌，可获得较好的菌细胞和Pb^2＋的去除效果。在浮选pH=6．25、十二胺用量为0．375mol／L时，菌细胞和Pb^2＋的去除率均达到了98％以上。选用Na2C03作为解吸剂，浓度为1．2mol／L、pH=4．75时，对吸附Pb“后的胶质芽孢杆菌的解吸效果可以达到100％。动电位测试和红外光谱分析结果表明，胶质芽孢杆菌的自然等电点为2．39，吸附Pb“后变为2．83，菌细胞与Pb^2＋的吸附过程主要与细胞多糖成分有关，吸附过程主要是静电吸引；吸附了Pb^2＋的菌细胞用十二胺浮选后等电点增至2．94，十二胺对菌细胞的浮选与细胞表面的酰胺基团和缔合-OH有关，吸附过程中有静电力、氢键和范德华力参与。

英文摘要：

The possibility of using bacillus mucilaginosus as the adsorbent to remove Pb^2＋ from water by biosorptionflotation process is studied, and the biosorption mechanism and the mechanism of the adsorbent and the collector are analyzed. The results indicate that cationic collector has a high removal rate of bacillus mucilaginosus. A good bacillus cell and Pb^2＋ removal effect can be achieved by floating Pb^2＋ -loaded bacillus mucilaginosus with dodecyl amine （DA） at a pH value between 3 - 6. When at a pH value of 6.25 and a DA dosage of 0. 375 mol/L, the removal rate can be above 98%. When 1.2 mol/L Na2CO3 is used as a desorbent at a pH value of 4. 70, the Pb^2＋ desorption rate from bacillus mucilaginosus can reach 100%. Zeta potential and infrared spectroscopy analysis results show the isoelectric point of bacil- lus mucilaginosus will change from the original 2.39 to 2.83 when it has adsorbed Pb^2＋. The adsorption between the bacillus cell and Pb^2＋ is mainly related to the cell＇s amylase component and the adsorption process is mainly of electrostatic attraction type. The isoelectric point of Pb^2＋ -loaded bacillus mucilaginosus increases to 2.94 when it is floated by DA, whose flotation for the bacillus cells is related to the acetylamino group on the cell surface and associating -OH. Electrostatic attraction, hydrogen bonding and Ven De Waals force are involved in the adsorption process.