脂肪及脂肪酸组分的改良是大豆油脂品质育种的主要方面。本研究旨在构建遗传图谱,定位大豆脂肪及脂肪酸组分的QTL,为大豆油脂品质育种提供参考。以Essex×ZDD2315的114个BC1F1单株为作图群体,构建了250个SSR标记和1个形态标记,具有25个连锁群的遗传图谱,覆盖大豆基因组2963.5cM,平均每个连锁群上10.0个标记,标记平均间距11.8cM。用BC1F3家系3个重复的表型平均值代表相对应的BC1F1单株表型值,采用Win QTLCartographer2.5复合区间作图法(CIM)检测到18个控制脂肪及脂肪酸组分含量的QTL,位于9个不同的连锁群上,表型贡献率为9.6%~34.5%;多区间作图法(MIM)检测到与CIM区间相同的7个QTL(fat-1、pal-1、st-1、ole-1、lin-1、lin-4和lio-2),区间相近的2个QTL(ole-4和lin-5),位于6个不同的连锁群上,表型贡献率为8.2%~39.3%。CIM法检测到的其他9个QTL有待进一步验证。大豆脂肪及脂肪酸组分含量的主效QTL数量不多,效应大的不多,可能还受许多未能检测出来的微效基因控制。育种中既要注意主效QTL...
Genetic improvement of fat and fatty acid composition content of soybean seed is interesting to both farmers and processors.The objective of the present study was to aim at constructing a genetic linkage map and mapping QTLs conferring fat and fatty acid composition contents in soybean.The mapping population with 114 BC1F1 plants of the soybean backcross(Essex×ZDD2315)×ZDD2315 was established and tested for their genetic variation,and then used to construct a genetic linkage map by using MAPMAKER 3.0.The ge...