中文摘要：

目的：建立多波长同时测定地锦草药材中没食子酸、鞣花酸、槲皮素、山柰酚4种指标成分含量的超高效液相色谱法,并比较不同地锦草化学成分的差异。方法：采用Acquity UPLC BEH C18色谱柱（50 mm×2.1 mm,1.7μm）,以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,柱温30℃,流速0.2 m L·min-1,检测波长为271 nm（0-2 min）、254 nm（2-6 min）和360 nm（6-8 min）。结果：地锦草中没食子酸、鞣花酸、槲皮素、山柰酚4种成分在8 min内均达到基线分离,线性范围分别为65.88-164.70（R2=0.999 2）、149.10-372.75（R2=0.999 2）、27.48-68.70（R2=0.999 7）、30.24-75.60μg·m L-1（R2=0.999 2）,平均回收率（n=6）分别为99.5%（RSD=1.4%）、98.3%（RSD=0.92%）、99.1%（RSD=1.2%）和99.3%（RSD=1.4%）;6批地锦草样品中没食子酸、鞣花酸、槲皮素、山柰酚的含量分别为4.148-7.663、9.727-17.388、1.763-2.977、0.684-2.037 mg·g-1。结论：本文对不同产地地锦草中指标性成分含量差异性的研究,可为地锦草的质量控制提供理论依据。

英文摘要：

Objective： To establish a multi-wavelength UPLC method for simultaneous determination of four index components （ gallic acid, spent acid, quercetin and kaempferol ） in Euphorbiae Humifusae Herba, and to compare the difference among samples from different habitants. Methods： The analysis was performed on a 30 ℃ thermostatic Acquity UPLC BEH Cls column （ 50 mm× 2.1 mm, 1.7 μm ）, with the mobile phase consisting of acetonitrile and 0.2% phosphoric acid by gradient elution. The flow rate was 0.2 mL · min^-1 and the detection wavelengths were 271 nm（ 0-2 min ）, 254 nm（ 2-6 min ） and 360 nm（ 6-8 rain ）. Results： Baseling resolved peaks were achieved for four components in 8 minutes. The linear ranges of gallic acid, spent acid, quercetin and kaempferol fell within the ranges of 65.88-164.70 μg·mL^-1（R2=0.999 2 ）, 149.10-372.75 μg·mL^-1 （ R2=0.999 2 ）, 27.48-68.70 μg·mL^-1 （ R2-0.999 7 ） and 30.24-75.60 μg·mL^-1（ R2=0.999 2 ）, respectively. The average recoveries （ n=6 ） were 99.5% （ RSD=1.4% ）, 98.3% （ RSD=0.92% ）, 99.1% （ RSD=1.2% ） and 99.3% （ RSD=1.4% ）, respectively. The contents of four components in 6 batches for samples were 4.148-7.663 mg·mL^-1, 9.727-17.388 mg·mL^-1, 1.763-2.977 mg·mL^-1and 0.684-2.037 mg· g^-1, respectively. Conclusion： This paper revealed difference in contents of indicative components in Euphorbiae Humifusae Herba, thus providing a theoretical basis for the quality control of the herb.