中文摘要：

瞄准：用 cDNA 在在正常怒气和门 hypertensive 怒气之间的微噬菌体(Mphi ) 的基因表示识别差别微数组并且发现新基因函数在门静脉高血压与脾机能亢进联系了。方法：包含 cDNAs 的 14112 个点的 Biostar-H140s 薄片被用来调查表示的差别。从从正常怒气和门孤立的巨噬细胞提取的全部的 RNA 高血压的怒气相对地与加入被抄录到 cDNA 荧光灯(cy3 和 cy5 ) 把 dCTP 标记准备杂交探针。在杂交以后，基因薄片为荧光灯的紧张被扫描。差别表示了基因被屏蔽。那被重复三次，和仅仅在三个薄片被考虑在门静脉高血压与脾机能亢进被联系的所有有微分表示的基因。结果：八 196， 1330 和 898 基因被识别是分别地在三个薄片表示的差别。121 基因(0.86%) 被识别是在所有三个薄片表示的差别，包括 21 起来调整的基因和 73 下面调整的基因。表示基因与离子的隧道和运输蛋白质有关的差别， cyclin，细胞骨架，房间受体，房间信号行为，新陈代谢，免疫者等等。这些基因可能与在门静脉高血压的脾机能亢进有关。结论：调查基于 cDNA，微数组罐头屏幕差别表示了在正常怒气和门 hypertensive 怒气之间的巨噬细胞的基因，可以因此在在门静脉高血压学习脾机能亢进的致病提供一个新想法。

英文摘要：

AIM： To identify the difference in gene expression of microphage （Mφ） between normal spleen and portal hypertensive spleen using cDNA microarrays and find new gene functions associated with hypersplenism in portal hypertension. METHODS： The Biostar-H140s chip containing 14112 spots of cDNAs were used to investigate the difference of the expression. The total RNA extracted from macrophages isolated from both normal spleen and portal hypertensive spleen was reversely transcribed to cDNA with the incorporation of fluorescent （cy3 and cy5） labeled dCTP to prepare the hybridization probes. After hybridization, the gene chip was scanned for the fluorescent intensity. The differentially expressed genes were screened. That was repeated three times, and only the genes which had differential expression in all three chips were considered to be associated with hypersplenism in portal hypertension. RESULTS： Eight hundred and ninety-six, 1330 and 898 genes were identified to be differentially expressed in three chips, respectively. One hundred and twentyone genes （0.86%） were identified to be differentially expressed in all three chips, including 21 up-regulated genes and 73 down-regulated genes. The differentially expressed genes were related to ionic channel and transport protein, cyclin, cytoskeleton, cell receptor, cell signal conduct, metabolism, immune, and so on. These genes might be related to the hypersplenism in portal hypertension. CONCLUSION： The investigations based on cDNA microarray can screen differentially expressed genes of macrophages between normal spleen and portal hypertensive spleen, thus may provide a new idea in studying the pathogenesis of hypersplenism in portal hypertension.