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A multiple-labelling method for cells using Au nanoparticles with different shapes
  • 期刊名称:Chinese Science Bulletin
  • 时间:2013.4.8
  • 页码:2640-2645
  • 分类:O484.41[理学—固体物理;理学—物理] TB383[一般工业技术—材料科学与工程]
  • 作者机构:[1]Chinese Academy of Sciences Key Laboratory of Standardisation and Measurement for Nanotechnology, National Centre for Nanoscience and Technology, Beijing 100190, China, [2]Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Centre for Nanoscience and Technology, Beijing 100190, China
  • 相关基金:This work was supported by the National Basic Research Program of Chi- na (2012CB933800 and 2011CB932802), and the National Natural Sci- ence Foundation of China (21173056 and 21201041).
  • 相关项目:纳米双相复合磁粉的原位制备及其热压/热变形研究
中文摘要:

一个重要、困难的问题同时在房间表面上正在识别各种各样的分子的详细地点,当这鉴定在一个生活房间要求在超过一个分子之间的 synergistic 效果。有不同形状的 Au nanoparticles (NP ) 能乐意地在扫描电子显微镜学(lvSEM ) 的低真空下面被认出。各向异性的 Au nanorods (NR ) 拥有唯一的表面电浆子回声(SPR ) 性质,它能进一步为二光子光(TPL ) 和另外的光成像被利用技术。在这份报纸, Au NR 和 Au nanooctahedra (Au NOs ) 为 ICAM-1 和 Integrin 1 作为 biomarkers 被介绍。与 lvSEM 的优点结合了,这个多重标记的方法是为学习在特定的、功能的分子之间的相互作用的一个新方法。

英文摘要:

An important and difficult issue is simultaneously identifying the detailed locations of various molecules on the cell surface, as this identification requires a synergistic effect between more than one molecule in a living cell. Au nanoparticles (NPs) with dif- ferent shapes can be readily recognised under low vacuum scanning electron microscopy (lvSEM). Anisotropic Au nanorods (NRs) possess unique surface plasmon resonance (SPR) properties, which can be further utilised for two photon luminescence (TPL) and other optical imaging techniques. In this paper, Au NRs and Au nanooctahedra (Au NOs) are introduced as biomarkers for ICAM-1 and Integrin β1. Combined with the advantages of lvSEM, this multiple-labelling method is a new method for study- ing the interactions between specific, functional molecules.

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