以不同生长来源的3种"宁杞1号"枸杞苗为试材,通过比较其生长状态及特点,筛选诱导愈伤组织形成的外植体来源枸杞苗。同时,研究了不同激素组合对枸杞愈伤组织形成的影响。在此基础上,比较了以叶片和茎段为外植体,愈伤组织形成的时间及长势。结果表明:"宁杞1号"枸杞种子种植的无菌苗长势好,继代后性状稳定,可作为最佳外植体材料来源;以叶片为外植体,诱导愈伤组织形成时间短、长势好、不定芽形成早;初步确定诱导枸杞叶片形成愈伤组织的最佳培养基为MS+0.2mg·L-1 NAA+1.0mg·L-1 6-BA。
The experiment has compared the growth and characteristics of‘Ningqi No.1'seedlings from three different kinds of sources,by which the best explant sources were found out.At the same time,effect on callus induction and their status under different combinations of exogenous hormones was also investigated.On this basis,callus formation from leaf and stem explants and their growth status were compared.The results showed that seedling from aseptic seeding grew well,and kept stably after subculture,which can be used as the best explants.Callus formation time from leaf explant was short.Besides,callus grew better and came into bud earlier.The optimum conditions for callus induction of wolfberry leaves were found out to be MS+0.2mg·L-1 NAA+1.0mg·L-1 6-BA.