中文摘要：

目的探讨孤儿核受体Nur77对氧化低密度脂蛋白（ox-LDL）诱导的血管平滑肌细胞增殖的影响。方法体外原代培养大鼠血管平滑肌细胞,在ox-LDL及其相关成分7-酮胆固醇（7-KC）、9-HODE和13-HODE等条件刺激下,应用实时荧光定量反转录聚合酶链反应（RT-PCR）技术、蛋白免疫印迹技术和免疫荧光染色结合激光扫描共聚焦显微镜技术检测Nur77 mRNA和蛋白表达变化。用腺病毒感染血管平滑肌细胞过表达Nur77,通过细胞计数和Western Blotting观察Nur77对血管平滑肌细胞增殖的影响。结果 ox-LDL可诱导血管平滑肌细胞中Nur77 mRNA表达上调,并且在1 h时达到高峰;同时ox-LDL及其相关成分（7-KC、9-HODE和13-HODE）,均可诱导血管平滑肌细胞中Nur77的蛋白表达明显上调。在ox-LDL刺激下,Nur77过表达组（Ad-GFP-Nur77）平滑肌细胞的细胞密度[（2.70±0.14）×104]及细胞增殖相关蛋白cyclin D1的表达明显低于对照组（Ad-GFP）平滑肌细胞的细胞密度[（3.95±0.26）×104]及cyclin D1的表达（P〈0.05）。结论孤儿核受体Nur77抑制ox-LDL诱导的血管平滑肌细胞增殖。

英文摘要：

Objective To investigate the role of orphan nuclear receptor Nur77 in oxidized low density lipoprotein（ ox-LDL）-induced vascular smooth muscle cells（ VSMCs） proliferation. Methods Primary rat VSMCs were cultured and stimulated with ox-LDL and its main lipid components（ 7-KC,9-HODE and13-HODE）. Nur77 expression was detected by real time PCR,Western blotting and immunofluorescence staining. To further investigate the role of Nur77 in VSMCs proliferation,primary rat VSMCs were infected with GFP-Nur77 adenovirus（ Ad-GFP-Nur77） and GFP control adenovirus（ Ad-GFP）,and VSMCs proliferation was detected using cell counting and Western blotting. Results Ox-LDL can induce vascular smooth muscle cells Nur77 mRNA expression to increase which peaked at 1 h; and induced by ox-LDL and its main lipid components（ 7-KC,9-HODE and 13-HODE） stimulation,VSMCs Nur77 protein levels were significantly up-regulated. By ox-LDL stimulation,Nur77 overexpression group（ Ad-GFP-Nur77） cell density[（ 2. 70 ±0. 14） × 104]and the expression of proliferation related cyclin D1 protein were significantly lower than the control group（ Ad-GFP） [smooth muscle cells density：（ 3. 95 ± 0. 26） × 104]（ P〈 0. 05）. Conclusion Nur77 inhibits ox-LDL-induced VSMCs proliferation.