利用10个微卫星标记对新疆巴什拜羊4个品系(红毛、白毛、黑毛和瘦肉型新品系)189个个体进行检测,分析群体遗传多样性和群体间的遗传分化、系统发育关系。结果表明:在巴什拜羊10个微卫星座位中共检测到110个等位基因,平均每个座位等位基因数为11个;4个群体10个微卫星标记的平均多态信息含量为0.791 4,平均杂合度为0.814 9,说明巴什拜羊4个群体均具有丰富的遗传多样性;4个绵羊群体的总近交系数为-0.178 2,群体内近交系数为-0.211 2,群体间基因分化系数为0.023 7,说明4个绵羊群体间2.37%的遗传变异来自群体间,而97.63%的遗传变异是由各群体内个体间的差异引起的;基因流(Nm)平均值为8.916 7。聚类分析发现,红毛品系与黑毛品系亲缘关系较近,之后与白毛品系相聚,最后与瘦肉型新品系聚在一起,聚类结果与品系育成史基本一致。
The four Bashibai sheep populations in Xinjiang,which contain 189 individuals,were detected using 10 microsatellite markers.And the genetic diversities,genetic differentiation and the phylogenetic relationship were analyzed in this study.The results showed that 110 alleles were detected in the 10 microsatellite markers of Bashibai sheep and the average number of allele was 11 in each locus.The average polymorphism information content was 0.791 4 in the 10 microsatellite markers of 4 sheep populations,and the average heterozygosity was 0.814 9,which showed that the 4 populations of Bashibai sheep have plenty of genetic diversities.The global heterozygote deficit across all populations was-0.178 2.The overall significant deficit of heterozygotes because of inbreeding within breeds was-0.211 2.The coefficient of genetic differentiation was 0.023 7,indicating that 2.37% of the total genetic variation could be explained by breed differences and remaining 97.63% by differences among individuals for each population.The average gene flow was 8.916 7.The cluster analysis showed that the genetic relationship between red hair strain and black hair strain was nearer,and then got together with white hair strain,and at last got together with new lean strain sheep.The cluster analysis was generally in accordance with their breeding history.