欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
基于分子吸收和发射测量，研究了盐酸小檗碱与特定序列DNA间的相互作用。结果发现双链DNA中含有不配对G碱基时，其与盐酸小檗碱作用后产生的光谱特征将明显不同于完全互补的双链DNA。该特征可被用于检测1个错配碱基的双链DNA。利用Hyperchem Professional软件包计算了4种寡聚核苷酸的电荷分布情况，并在此基础上进一步探讨了盐酸小檗碱与寡聚核苷酸之间的作用机理。
英文摘要：
The interaction of berberine hydrochloride （BBH） with sequence-specific DNA was studied by measuring their molecular absorption and fluorescence spectra. It was found that the characteristic spectrum produced by the interaction of BBH with double-bond DNA containing the unpaired G bases was significantly different from that with the complementary double-bond DNA. The characteristic spectrum could be used to detect the double-bond DNA with one base mispairing. Based on the charge distribution of four kinds of oligonucleotides calculated with the software package of Hyperchem Professional, the mechanism of the interaction of BBH with oligonucleotides was further revealed.
同期刊论文项目
同项目期刊论文
Tryptophan-contained peptide-functional nanomaterials as general spectrofluorometric reagents for en
Adsorption of penicillin–berberine ion associates at a water/tetrachloromethane interface and determ
Selective (poly T) Recognition of Thymidine Homopolymer Oligonucleotide with Cobalt(II)/4-[(5- Chlor
A quantitative method for determination of Co(II) based on the inner filter effect of reagents on th
Immunoassay by detecting enhanced resonance light scattering signals of immunocomplexes using a comm
Spectral assignments to the light emissions of fluorescent organic small molecules in aqueous medium
A coupled reagent of o-phthalaldehyde and sulfanilic acid for protein detection based on the measure
A backscattering light detection assembly for sensitive determination of analyte concentrated at the
A wide dynamic range detection of biopolymer medicines with resonance light scattering and absorptio
Determination of heparin using azure B by flow Injection analysis-resonance light scattering coupled
Label-free detection of sequence-specific DNA with multiwalled carbon nanotubes and their light scat
Conformational change detection of DNA with the fluorogenic reagent of o-phthalaldehyde- (-mercaptoe
A one-step label-free optical genosensing system for sequence-specific DNA related to the human immu
Hybridization detection of DNA by measuring organic small molecules magnified resonance lights catte
A dual-wavelength resonance light scattering ratiometry of biopolymer by its electrostatic interacti
A one-pot green method for one-dimensional assembly of gold
nanoparticles with a novel chitosan-ninh
Light-scattering signals from nanoparticles in biochemical assay, pharmaceutical analysis and biolog
A label-free visual immunoassay on solid-support with silver nanoparticles as plasmon resonance scat
A resonance light scattering ratiometry applied for binding study of organic small molecules with bi
Directly light scattering imaging of the aggregations of biopolymer bound chromic hydrolytic oligome
A novel optical immunosensing system based on measuring surface enhanced light scattering signals of
Spectral studies on the interaction of amino black 10B with DNA in the presence of cetyltrimethylamm
On the factors affecting the enhanced resonance light scattering signals of the interactions between
Selectively light scattering spectrometric detection of copper (II) based on a new synthesized oxami
Pharmacokinetic detection of penicillin excreted in urine using a totally internally reflected reson
期刊信息
位置：