中文摘要：

目的 明确半乳糖凝集素-1（galectin-1）在脐带间充质干细胞（UC-MSCs）调控RA成纤维样滑膜细胞（FLS）功能中的作用。方法用慢病毒构建galectin-1低表达的UC—MSCs，即UC-MSCs（Gal-1-）。采用Transwell非细胞接触方式分别将UC-MSCs及UC-MSCs（Gal-1-）和FLSs共培养。MTS法检测FLSs增殖，将基质胶铺在Transwell板中来检测UC-MSCs（Gal-1-）对FLSs侵袭能力的影响。多组间计量资料采用单因素方差分析。结果UC-MSCs及UC-MSCs（对照shRNA）．FLSs共培养组可明显抑制TNF-α诱导的FLSs的增殖作用，而UC-MSCs（Gal-1-）对此增殖无明显抑制作用。UC-MSCs及UC-MSCs（对照shRNA）可显著抑制TNF-α引起的FLSs侵袭细胞数（37±7，42±10与55±14，F=23．50，P〈0．05），但UC-MSCs（Gal-1-）-TNF组与TNF-α刺激组相比，侵袭细胞数差异无统计学意义（46±11，P〉0．05）。结论UC-MSCs可抑制RA患者FLS的增殖及侵蚀能力，但敲低galectin-1分子的表达后该作用减弱，提示galectin-1参与了UC-MSCs抑制FLS功能的过程。

英文摘要：

Objective To investigate the effects of galectin-1 on regulating of umbilical cord mesenchymal stem cells （UC-MSCs） of rheumatoid arthritis （RA） fibroblast-like synoviocytes （FLSs）. Methods Lentivirus transfected shRNA technique was used to knock down the expression of galectin-1 in UC-MSCs to construct UC-MSCs （Gal-1-）. The effects of UC-MSCs and UC-MSCs （Gal-1-） on FLSs in RA patients were investigated by transwell system. The proliferation of FLSs was detected by MTS assay. The effect of UC-MSCs on the invasion of FLSs was detected by matrix gel in transell plate. Measurement data were analyzed with oneway analysis of variance. Results In vitro, UC-MSCs were capable of inhibiting tumour necrosis factor-a （TNF-a） induced proliferation of FLSs from RA patients, but UC-MSCs （Gal-1-） did not show significant inhibitory effect. Furthermore, the invasive behavior of FLSs was also significantly suppressed by UC-MSCs （37e7, 42±10, 55±4, F=23.50, P〈0.05）. These was no significant difference in the number of invasive cells between TNF-ot group and UC-MSCs （Gal-1-）-TNF-ot group. Conclusion UC-MSCs can inhibit the proliferation and invasive behavior of FLSs from RA patients, but these effects decline after knocking down the expression of galectin-1. Galectin-1 may take part in the regulation of UC-MSCs on rheumatoid arthritis FLSs.