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中文摘要：
用荧光法探索了十六烷基三甲基溴化铵（CTMAB）、十二烷基硫酸钠（SDS）、β-环糊精（β-CD）、聚氧乙烯月桂醚（Brij-35）4种有序介质,及反应时间、pH等条件对槲皮素-Al 3＋体系荧光强度的影响。结果表明：四种有序介质中,CTMAB在酸性介质（pH=6.10）下对槲皮素-Al 3＋体系荧光增敏效果最好,在λex/λem=450/539nm处有强荧光峰。据此,建立了一种用CTMAB增敏荧光光度法测定总黄酮含量的新方法,其线性范围为4.0×10-8~1.0×10-5 mol/L,检出限为1.24×10-8 mol/L,相对标准偏差（RSD）为2.26%。方法已用于银杏叶片中黄酮含量的测定。
英文摘要：
The effects of four kinds of order media including cetyltrimethylamine bromide（CTMAB）,sodium dodecyl sulfate（SDS）,β-cyclodextrin（β-CD）,polyethylene lauryl ether（Brij-35）,as well as the reaction time and pH on the fluorescence of quercetin-Al 3＋system were studied with spectrofluorimetry.The experiments showed that the CTMAB had the best sensitizing effect among four kinds of order media for the fluorescence of flavonoids in quercetin-Al 3＋system at acidic conditions.A method is accordingly established with CTMAB as a fluorescence sensitizing agent for determining total flavonoids content.The linear range was 4.0×10-8-1.0×10-5 mol/L with the detection limit of 1.24×10-8 mol/L and RSD% of 2.26%.The method has been used for determining total flavonoids content in Ginkgo bilobaleaf.
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