以4种不同温度类型的冬小麦品种小偃6号、NR9405、陕229和RB6为材料,在0~250mmol/LNaCl胁迫条件下,采用水培试验研究了钙调蛋白抑制剂(CPZ)、钙离子通道阻断剂(LaNO3)和钙离子螯合剂(EGTA)等对冬小麦盐分吸收的影响及其作用机理.结果显示:在中盐(150mmol/LNaCl)胁迫下3种制剂均显著增加了各品种小麦植株对Na+的吸收,减少了K+吸收,且其作用依次表现为CPZ〉EGTA〉LaNO3.中盐胁迫下,各种制剂对冷型小麦陕229和RB6盐分吸收的影响大于暖型小麦小偃6号和NR9405,而高盐(250mmol/L)胁迫下这种差异缩小.研究表明,Ca^2+对小麦Na^+吸收的调节作用主要通过钙调蛋白(CaM)来完成,CaM不但控制小麦Na+净吸收和累积,而且在小麦K^+吸收中也起重要作用;在盐胁迫下冷型小麦陕229和RB6对Ca^2+和CaM活性的要求大于暖型小麦小偃6号和NR9405.
Under 0-250 mmol/L NaCl concentrations, hydroponic experiments were conducted to investigate the effect of calmodulin inhibitor (chlorpromazine,CPZ), Ca^2+ channels inhibitor (LaNO3) and Ca^2+ chelator (EGTA) on sodium and potassium uptake and its' mechanism with 4 winter wheat varieties, 'Xiaoyan 6', 'NR9405', 'Shaan 229' and 'RB6' ,which are different in canopy temperature. The results indicated that under medium (150 mmol/L,NaCl) salt concentration, sodium uptake is promoted significantly, and the potassium uptake is slowed down by 3 inhibitors. And the effect of inhibitors is as follow:CPZ〉 EGTA〉LaNO3. So the function of Ca^2+ on sodium uptake mainly by way of combination of Ca^2+ and calmodulin (CAM). The calmodulin not only regulate Na^+ efflux,which is critical to net Na^+ influx in root cells and accumulation in the shoot, but also regulate K^+ influx. Under medium salt concentration (150 mmol/ L,NaCl),the effect of inhibitors or chelator on the Na^+ uptake of 'Shaan 229' and 'RB6' is higher than that of 'Xiaoyan 6 ' and 'NR9405 ' , but there is little difference among the different genotypes under higher salt stress (250 mmol/L NaCl). So that 'Shaan 229' and 'RB6' are more sensitive to lower Ca^2+ and CaM activity than that of 'Xiaoyan 6' and ' NR9405 '.