中文摘要：

目的：建立高效液相色谱-荧光检测测定大鼠血小板内5-羟色胺含量的方法。方法：色谱柱为Nucleosil-C18（4 mm×250 mm，10 μm），流动相：磷酸二氢钾（10 mmol/L，内含EDTA-Na2 0.3 mmol，pH=3.5）∶甲醇∶乙腈＝85∶10∶5，流速：0.5 ml/min，荧光激发波长：254 nm，发射波长：360 nm，增益：32。血小板沉淀用300 μl纯水悬浮，加入15 μl内标溶液（30 μg/ml，α-甲基-5-HT水溶液），100 μl蛋白沉淀液，混匀，离心，取上清液20 μl，进样。结果：5-羟色胺在0.25～4.00 μg/ml浓度范围内，线性关系良好。5-羟色胺测定的批内和批间相对标准差（RSD）分别小于4.29％和4.44％，回收率为84.3％～88.7％。正常雄性SD大鼠血小板内5-羟色胺的平均含量分别为（1.65±0.14） μg/109 plts。结论：本法简便、准确、快捷，可用于大鼠血小板内5-羟色胺的测定。

英文摘要：

Objective： To establish a method for the contend determination of serotonin in blood platelets by high performance liquid chromatography with fluorescence detector （HPLC-FD）. Methods： Preparating platelets samples commonly with 2% EDTA-Na2 anticoagulated blood, we determinated them by HPLC. The nucleosil-Cls column （4 mm×250 mm, 10 μm） was used. The mobile phases consisted of mobilphase of 0.01mol/L-potassium dihydrogen phosphate, acetonitrile and methanol （85：5：10）, the flow rate was 0.5 ml/min and the detection wavelength was at 254 nm, the excitation wavelength was at 360 nm for emission, quantitating in internal standard method. Results： The results showed that the 5-Hydroxytryptamine peak was chromatographically resolved from the internal satandard; a good linearity range from 0.25-4.00 μg/ml; the RSD of within- batch and between-batch of the method was 4.29% and 4.44%; the average recovery was 84.3%-88.7%. The average contents of 5-Hydroxytryptamine in platelets of normal male Sprague-Dawley rats was （1.65±0.14） μg/109 pits. Conclusion： The method is simple, accurate, rapid, and can be used for the determination of 5-Hydroxytryptamine in rates platelets.