中文摘要：

几乎所有蜈蚣都是肉食动物，都依靠其毒液来捕杀和消化猎物，蜈蚣粗毒为混合物，其主要成分为蛋白质．本文以蜈蚣粗毒为原料，通过反相高效液相色谱进行分离纯化，将纯化产物进行MALDI-TOF—MS鉴定，发现蜈蚣粗毒中具有多肽组分．收集多肽组分在DRG细胞上进行全细胞膜片钳电生理实验研究，发现其中4个组分具有抑制电压门控钠通道电流活性、4个组分具有抑制电压门控钾通道电流活性．表明蜈蚣毒素具有很好的开发应用前景，为后续工作打下了良好的实验基础．

英文摘要：

Almost all centipedes are predators and have venom glands. The primary purpose of centipede venoms is to kill or paralyze prey. Centipede venoms are complex chemical cocktails in which proteins are the main components. This study, investigated the biochemical and pharmacological properties of the venom of the centipede Scoropendra subspinipes mutilans Koch The RP-HPLC and MALDI-TOF MS spectra of the venom indicated that it contains diverse peptides with molecular weights from 3000-10000 Da. Using the whole-cell patch-clamp, it was found that the venom could inhibit both voltage-gated sodium channels （VGSC） and potassium channels （VGPC） in adult rat dorsal root ganglion （DRG） neurons. Furthermore, pharmacological activities of the 43 fractions collected from RP-HPLC were examined on both VGSCs and VGPCs in rat DRG neurons. Consequently, we identified four fractions containing peptide neurotoxins which could inhibit VGSCs, as well as the other four fractions that containing peptide neurotoxins which could inhibit VGPCs. This study indicates that the venom of the centipede Scoropendra subspinipes mutilans Koch is indeed a rich source of novel pharmacologically interesting peptides that would receive increased attention from pharmacologists and biochemists in the future.