为降低薏米糠油酸值,提高其品质,采用响应面法优化了薏米糠油的碱炼脱酸工艺条件,并研究了精炼薏米糠油的活性。结果表明:薏米糠油碱炼脱酸最佳工艺条件为超量碱0.21%、碱炼时间33 min、碱液质量分数26.58%、碱炼温度58℃,在最佳工艺条件下薏米糠油酸值(KOH)从45.9 mg/g降到0.59 mg/g;精炼薏米糠油在质量浓度0.75-12 mg/m L范围内对酪氨酸酶的抑制率表现出良好的剂量依赖关系,IC_(50)为2 mg/m L;同时精炼薏米糠油对DPPH自由基的清除能力IC_(50)为6.36 mg/m L,表明其具有一定的美白、抗氧化功效。精炼薏米糠油是一种高品质、高生物活性的天然植物油,有望开发为功能性油脂产品。
In order to improve the quality and reduce the acid value of adlay bran oil, the alkali refiningdeacidification conditions of adlay bran oil were optimized by response surface methodology, and the ac-tivity of refined adlay bran oil was studied. The results showed that the optimal alkali refining deacidifica-tion conditions of adlay bran oil were obtained as follows: excessive alkali 0.21%, alkali refining time33 min, mass concentration of alkali solution 26.58%, and alkali refining temperature 58 ℃. Underthese conditions, the acid value of adlay bran oil reduced from 45.9 mgKOH/g to 0.59 mgKOH/g. Be-sides, the refined adlay bran oil had dosage -dependant relationship with inhibitory rate of tyrosinase inthe mass concentration of 0.75 - 12 mg/mL, and the IC50 was 2 mg/mL. The IC50 of refined adlay branoil against DPPH radical was 6.36 mg/mL. It turned out that adlay bran oil had certain whitening effectand antioxidant activity. In a word, refined adlay bran oil was a kind of natural vegetable oil with highquality and biological activity, which might be developed as functional oil in the future.