中文摘要：

目的探讨体外补充不同剂量叶酸对HPV阴性细胞C33A和HPV16阳性细胞CaSki的细胞增殖及凋亡的影响以及叶酸与HPV16的相互关系。方法倒置显微镜下观察叶酸对两种细胞基本生长状态的改变及形态的变化；采用MTT法和流式细胞术观察不同浓度叶酸（0．1、1．0、10、50、100、500、1000和2000μg／m1）对两种宫颈癌细胞的增殖抑制、细胞周期及凋亡情况；采用RT—PCR技术检测CaSki细胞中HPV16E2／E6mRNA的表达水平。结果补充叶酸对C33A和CaSki细胞的增殖均有抑制作用，细胞形态发生改变，细胞数量明显减少。随着叶酸浓度的增加，对两种宫颈癌细胞的生长抑制率逐渐上升（C33A：r=0．948，P=0．010；CaSki：r=0．895，P=0．006），G0／G1期细胞比例逐渐增大，细胞凋亡率亦随之升高（C33A：r＝0．989，P〈0．001；CaSki：r=0．994，P〈0．001），呈现线性关系。但对C33A和CaSki两种细胞的增殖抑制及凋亡的影响程度无明显差异。当补充叶酸时，CaSki细胞HPV16E2和E6基因mRNA的表达水平在对照组、叶酸缺乏组和叶酸实验组之间的差异无统计学意义。结论补充叶酸可抑制宫颈癌细胞的增殖，促进凋亡；未发现在宫颈癌细胞中叶酸与HPV16存在交互作用。

英文摘要：

Objective To study the effect of different concentration of folate in vitro on the proliferation and apoptosis of C33A cell with HPV negative and CaSki cell with HPV16 positive, and intreaction of folate and HPV16. Methods C33A and Caski cells were intervened by different concentration of folate （0.1, 1.0, 10, 50, 100, 500, 1 000 and 2 000 μg/ml）. The morphological changes of cells were examined by reverse discrepancy microscope. MTT and Flow Cytometry （FCM） were employed to measure the proliferation, inhibition ratio, cell cycle and apoptosis of cells. RT-PCR was used to detect the expression of HPV16 E2/E6 oncogene. Results The effects of folate on the C33A and CaSki cell growth suppression were increased gradually with the concentration of folate increasing and cultured duration prolonging, the inhibition ratio was increased （C33A：r =0.948, P =0.010; CaSki：r =0.895, P =0.006）. Adding folate could induce cellapoptosis （C33A： r=0.989, P〈0.001; CaSki： r =0.994, P〈0.001）, and there was a linear relationship, but there was no significant difference on the proliferation and apoptosis .of C33A and CaSki cells effected. In addition, concentration of folate did not affect the expression of HPV16 E2/E6. Conclusion Different concentration of folate inhibited cervical cancer cell proliferation and enhanced cell apoptosis, but the results do not support an interaction between folate and HPV16 in cervical cancer cell.