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中文摘要：
为了对一起死亡率高达91．3％、急性死亡的鸿雁病例进行病原学分析，通过细菌分离排除法和病毒分离方法获得致鸭胚死亡病毒（暂命名为FJ-017株）。该病毒无血凝活性，用鹅细小病毒、番鸭细小病毒、鸭呼肠孤病毒、鸭甲肝炎病毒1型、鸭瘟病毒、鸭坦布苏病毒特异引物分别进行扩增，电泳结果显示，仅鸭甲肝炎病毒1型引物可扩增出条带。将扩增产物回收后克隆，序列分析表明FJ-017株与22株DHAV-1参考株的同源率为93．5％-99％，而与DHAV-2和DHAV-3参考株的同源性均为79．9％。遗传进化分析表明FJ-017株与DHAV-1关系密切，在进化树中共处一分支，表明FJ-017株为鸭甲肝炎病毒1型，此为国内外首次报道。
英文摘要：
The outbreak of an acute disease caused 91.3% fatality in a swan goose flock in Fujian Province. A Duck hepatitis A virus （DHAV） was isolated from a dead swan goose and designated as FJ-017 strain. The isolated FJ-017 strain had no hemagglutination activity. The genome of FJ-017 strain was amplified in PCR using specific primers targeted for DHAV-1 but not for Goose parvovirus, Duck parvovirus, Duck reovirus, Duck enteritis virus and Duck tembusu virus. Sequence analysis revealed high homology （93.5%-99%） between F J-017 strain and 22 DHAV-I strains in GenBank. The relatively lower homology （79.9%）, however, existed between F J-017 strain and two reference strains of DHAV-2 and DHAV-3. Phylogenetic analysis showed that F J-017 strain and DHAV-1 reference strains belonged to a distinct cluster. This was the first isolation of DHAV-1 virus from a swan goose.
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