中文摘要：

目的：建立苦地丁药材的超高效液相色谱指纹图谱。方法：采用UPLC-PDA技术,测定10批不同产地的苦地丁药材。Phenomenex Luna C18柱（250 mm×4.6 mm,5μm）,乙腈-0.02%三乙胺溶液梯度洗脱,流速0.3m L/min,柱温30℃,检测波长289 nm。结果：建立了10批不同产地的苦地丁药材的超高效液相色谱指纹图谱及其对照图谱。确定了13个共有峰,指认了其中的3个峰,各指纹图谱相似度大于0.80;聚类分析将不同产地药材分为3类;主成分分析得到3个主成分,累计方差贡献率达到89.607%,并判别出苦地丁药材中的2个主要化学成分。结论：该方法实现了苦地丁药材指纹图谱的快速、高效测定,为全面控制其质量提供了依据。

英文摘要：

Objective：To establish an Ultra Performance Liquid Chromatography fingerprint of Corydalis bungeana from different habitats. Methods：UPLC-PDA was adopted to analysis ten batches of Corydalis bungeana from different habitats with Phenomenex Luna CIs column（250 mm ×4. 6 mm,5μm）eluted with the mobile phase of acetonitrile and 0. 02% triethylamine in a gradient mode. The flow rate was 0. 3 mL/min and the column temperature was 30℃. The detection wavelength was set at 289 nm. Results ： The fingerprints of ten batches of Corydalis bungeana from different habitats had 13 common peaks ,three of them were identified. The similarities were larger than 0. 80. Ten batches of samples were divided into three categories by cluster analysis. Three principal components were observed via principal component analysis and the value of three principal components accounted for 89. 607% of the total variance. Two nlajor chemical components of Corydalis bungeana were confirmed. Conclusion：The high-performance and rapid method is successfully used for fingerprint analysis and can be used to evaluate the quality of Corydalis bungeana.