中文摘要：

用全细胞膜片钳方法研究纳米氧化铜（nanoCuO）颗粒对大鼠海马CA1区急性分离神经元延迟整流钾通道电流（Ik）的影响，并用MTT方法研究其对神经生长因子（NGF）诱导分化的PC12细胞活性的影响。结果显示5×10^-5g／mL nanoCuO能够显著抑制海马CA1区神经元的Ik，使其失活曲线和对照组相比向左偏移，但对其激活过程无明显影响。MTT方法的实验结果显示不同浓度的nanoCuO（5×10^-4、5×10^-5、5×10^-6g／mL）对NGF诱导分化的PCI2细胞均有损伤作用，随着浓度的增加，损伤更加明显，呈量效和时效依赖关系。

英文摘要：

The effects of nanoparticles of CuO on delayed rectifier potassium current （Ik） were studied in acutely isolated CA1 pyramidal neurons of rat hippocampus by using the whole-cell patch-clamp techniques and the effects on differentiated PC12 cell viability were studied with MTT assay. Nanoparticles of CuO mainly inhibited Ik in the concentration of 5×10^-5 g/mL. Nanoparticles of CuO didn＇t shift the steady-state activation curve of Ik but negatively shift the inactivation curve of Ik. Nanoparticles of CuO （5×10^-4,5×10^-5 and 5×10^-6g/mL） induced differentiated PC12 cells damage with dose-and time-dependent manner.