中文摘要：

目的：比较研究大鼠局灶性脑缺血再灌注损伤后细胞周期蛋白依赖性激酶抑制因子p15^ink4b在神经元和星形胶质细胞的表达。方法：建立大鼠大脑中动脉阻塞（MCAO）再灌注模型，应用流式细胞术检测各组MCAO再灌注后不同时期神经元和星形胶质细胞中的p15^ink4b的表达。结果：缺血侧皮质区星形胶质细胞中的p15^ink4b的表达在再灌注3d后开始下调，14d显著下调，与假手术组比较有显著性差异（P〈0．05）；缺血侧皮质神经元中的p15^ink4b在再灌注14d后表达下调，与假手术组比较有显著性差异（P〈0．05）。结论：局灶性脑缺血再灌注损伤后，缺血侧皮质区星形胶质细胞和神经元均有p15^ink4b不同程度的表达下调，星形胶质细胞中的p15^ink4b表达下调比神经元更为显著。

英文摘要：

Objective：The change of expression of p15^ink4b on astrocytes and neurons of rats after focal cerebral ischemia has been stuided. Methods： Ischemia was induced by temporary middle cerebral artery occlusion （MCAO）. The experimental rats induced by MCAO were sacrificed on days 1, 3, 7 and 14 after reperfusion. The brain was taken at days 1, 3, 7, 14 after injury, then the p15^ink4b of astrocytes and neurons was detected by flow cytometry. Results： Compared with that of the controls, the expression of p15^ink4b on astrocytes was remarkably down-regulated on the ischemic side of cerebral cortex 3, 7 and 14 days after reperfusion （ P〈 0.05）, Similarly, p15^ink4b on neurons was significantly down-regulated on the ischemic region 14 days after cerebral ischemia （ P〈 0.05）, but lesser than that on the astrocytes （ P〈 0.05）. Conclusion： Astrocytes and neurons show a different down-regulated expression of p15^ink4b after MCAO in rats. The p15^ink4b expression on astrocytes was more remarkably down-regulated than that on neurons.