中文摘要：

目的 观察不同碘营养水平下大鼠妊娠期甲状腺和胎盘胰岛素样生长因子（IGF）-Ⅰ及转化生长因子（TGF）-β1 mRNA表达水平的变化.方法 雌性Wistar大鼠150只,体质量80～100g,按体质量随机分为5组,每组30只,分别饮用含碘50（对照组,NI）、0（低碘1组,LI1）、5（低碘2组,LI2）、3000（高碘1组,HI1）、10 000 μg/L（高碘2组,HI2）的去离子水.饲养12周将雌鼠同雄鼠合笼交配,分别于孕早期（第6、7天）,孕中期（第12、13天）、孕晚期（第19、20天）处死,取甲状腺及胎盘.利用实时荧光定量PCR方法测定大鼠甲状腺和胎盘的IGF-Ⅰ及TGF-β1 mRNA表达水平.结果 ①LI1组和LI2组甲状腺绝对质量[（12.17±5.41）×10-2、（3.54±1.21）×10-2g]均高于NI组[（2.05±0.50）×10-2 g,P均＜0.05]；HI1组、HI2组甲状腺绝对质量[（1.64±0.27）×10-2、（1.66±0.29）×10-2 g]与NI组比较,差异无统计学意义（P均＞0.05）.②大鼠甲状腺IGF-Ⅰ mRNA表达：孕早期,LI1组、LI2组（1.98±0.35、1.47±0.22）均高于NI组（1.01±0.18,P均＜0.01）,HI1组、HI2组（0.68±0.16、0.75±0.09）均低于NI组（P均＜0.05）；孕中期,HI2组（1.14±0.17）低于NI组（1.58±0.33,P＜ 0.01）；孕晚期,LI2组、HI2组（1.47±0.20、1.45±0.35）均低于NI组（2.20±0.37,P均＜0.01）.NI组,孕早期、孕中期、孕晚期的IGF-Ⅰ mRNA表达水平（1.01±0.18、1.58±0.33、2.20±0.37）呈增高趋势,任意两组间比较差异均有统计学意义（P均＜ 0.01）.③大鼠甲状腺TGF-β1 mRNA表达：孕早期,H1组（1.37±0.13）高于NI组（1.05±0.18,P＜ 0.01）,HI1组、HI2组（0.50±0.09、0.44±0.11）均低于NI组（P均＜0.01）；孕中期,LI1组LI2组（1.39±0.28、1.17±0.12）均高于NI组（0.63±0.22,P均＜0.01）；孕晚期,LI1组、LI2组（1.57±0.30、1.23±0.20）均高于NI组（0.68±0.17,P均＜0.01）.NI组孕中期、孕晚期（0.63±0.22、0.68±0.17）均低于孕早期（1.05±0.18,P均＜0.01）.④大

英文摘要：

Objective To study the mRNA expression of rat Insulin-like growth factors- Ⅰ （IGF- Ⅰ ） and Transforming growth factor-β1 （TGF-β1） in thyroid and placenta with different iodine intakes during pregnancy.Methods One hundred and fifty female Wistar rats,weighting 80 - 100 g,were randomly divided into five groups according to body weight,30 rats in each group.Each group was given deionized water containing different concentrations of iodine,50 μg/L（control group,NI）,0 μg/L（iodine deficiency 1 group,LI1 ）,5 μg/L（iodine deficiency 2 group,LI2）,3000 μg/L（iodine excess 1 group,HI1 ）,and 10 000 μg/L（iodine excess 2 group,HI2）,respectively.After feeding for 12 weeks,the female rats were mated with male rats.The female rats were sacrificed at first（6,7 days）,trimester（ 12,13 days）,and third trimesters（ 19,20 days）,respectively,then their thyroid and placenta were collected.The mRNA expressions of IGF- Ⅰ and TGF-1 in thyroid and placenta were detected by real-time quantitative PCR.Results ①The actual thyroid weights of LI1 and LI2 groups[ （12.17 ± 5.41 ） × 10-2 g,（3.54 ± 1.21） × 10-2 g] were significantly higher than that of NI group[ （2.05 ± 0.50） × 10-2 g,all P 〈 0.05] ;actual weights of HI1 and HI 2 groups[ （1.64 ± 0.27） × 10-2 g,（1.66 ± 0.29） × 10-2 g] were compared with that of NI group,the difference was not statistically significant（all P 〉 0.05）.②The mRNA expression of IGF- Ⅰ： at the first trimester,LI1 and LI2 groups（l.98 ± 0.35,1.47 ± 0.22） were all higher than that of NI group（1.01 ± 0.18,all P〈 0.01 ）,HI1 and HI2 groups（0.68 ± 0.16,0.75 ± 0.09） were lower than that of NI group（all P 〈 0.01 ）;at the second trimester,HI2 group（ 1.14 ± 0.17） was lower than that of NI group（ 1.58 ± 0.33,P 〈 0.01 ） ; at the third trimester,LI2 and HI2 groups（1.47 ± 0.20,1.45 ± 0.35） were lower than that of NI group（2.20 ± 0.37,all P〈0.01）.The mRNA expression of IGF- I level in NI group at