中文摘要：

（R）-α-羟基苯乙酸是重要的药物合成中间体，本文研究Bacillus sp．HB20在含离子液体（[bmim]Cl）体系中催化合成（R）-α-羟基苯乙酸。首先采用Plackett-Burman试验设计法筛选出3个主要因素：离子液体浓度、反应温度、底物浓度。然后用最陡爬坡方法逼近最大响应区域，再用Box-Behnken试验设计和响应面分析法进行回归分析，确定出3个主要影响因素的最佳生产条件为：离子液体含量34．3g/L、反应温度33℃、底物浓度15.3mmol／L。得到的最大生产产率为82．39％，比优化前提高了21．66％。结果表明采用响应面方法有效地提高了（R）-α-羟基苯乙酸的产率，为手性药物的工业化生产提供了1条新的绿色途径。

英文摘要：

（R）-α-Hydroxyphenylacetic acid （（R）-HPA） was an important drug intermediates. In the current study, Bacillus sp.HB20 was used to catalyze the reduction of phenylglyoxylic acid （PGA） to （R）-HPA in the system containing ionic liquid （[bmim]Cl） and response surface methodology was successfully used to optimize the production of （R）-HPA. A significant influence of ionic liquid （IL） content, reaction temperature, and PGA concentration on （R）-HPA production was noted with Plackett-Burman design and the steepest ascent was applied to approach the optimal response area. Then, a three-level Box-Behnken design was employed to optimize the culture conditions for the production of （R）-HPA. The optimal conditions to achieve the optimal （R）-HPA production were determined： IL content, 34.3 g/L; reaction temperature, 33 ℃; PGA concentration, 15.3 mmol/L. The maximum production of （R）-HPA was 82.39 %, which was about 21.66 % increase compared with that obtained from the original culture conditions. It can be concluded that using response surface methodology to improve the （R）-HPA yield is available. It provides a new green method for the industrial production of chiral drugs.