中文摘要：

以松散型玉米自交系沈137为材料，利用同源克隆法成功克隆与水稻Dwarf4基因同源的玉米Dwarf4基因。序列分析发现，该基因cDNA序列全长1626bp，开放阅读框1521bp，编码506个氨基酸，与高粱、水稻、拟南芥的氨基酸同源性分别为95％、89％和71％。Real—TimePCR分析表明，ZmDwarf4基因的表达量为3～11叶期表达量逐渐增加，9～11叶期达到最大量，12．19叶期表达量逐渐降低，推测ZmDwarf4基因正向调控玉米叶夹角的大小。ZmDwarf4基因的表达量在叶片中最高，在叶枕中最低。不同激素处理的结果表明，用植物激素（IAA）处理的样品ZmDwarf4基因的表达量始终高于对照；用Brassinolide（BR）处理的样品6叶期ZmDwarf4基因的表达量高于对照，7-10叶期该基因的表达趋势与对照基本一致，但始终低于对照；用IAA＋BR处理的样品，该基因的表达趋势与对照基本一致，但高于对照。ZmDwarf4参与玉米BR生物合成途径，进而调控其相关株型建成。

英文摘要：

The Dwarf4 gene in maize which was homologous to the Dwarf4 gene in rice, and that was successfully cloned from inbred line Shen137 by homology cloning. Sequence analysis indicated that the full-length cDNAs was composed of 1 626 bp sequences including an open reading frame （ORF） of 1 521 bp region which encoded 506 amino acids. The ZmDwarf4 gene shared an amino acid sequence homology of 95% with sorghum, 89% with rice, and 71% with A rabidopsis thaliana. Real-Time PCR analysis showed that the expression level of ZmDwarf4 gene gradually in- creased from 3-leaf to 1 1-leaf stages with the highest expression level at 9-leaf to 1 1-leaf stage, the expression was gradually reduced from 12-leaf to 19-leaf stages. The expression pattern was in accordance with and the leaf angle variation of Shen137 in the period of growth and development, inferring that the ZmDwarf4 gene positive regulation the leaf angle of maize; As for the different organs, the expression level of the gene was the highest in leaf blade and lowest in pulvinus. The results of different phytohormone treatments showed that the expression of ZmDwarf4 gene of the sample which was treated by IAA was always higher than that of the control; the expression trend of BR processing samples was consistent with the control from 7-leaf to 10-leaf stages but always lower than that of the control, except that at 6-leaf stage; and the treatment with IAA＋BR samples, the gene expression trend were basically the same with the control＇, but were higher than the control. ZmDwarf4 involved in BR biosynthesis pathway of maize to regulate the plant morphogenesis.