中文摘要：

目的检测人胎膜组织中E26转录因子1（Ets-1）的表达及定位，探讨其与胎膜早破发生的关系。方法选择2007年2—11月在重庆医科大学附属第一医院住院分娩的产妇100例为研究对象，按足月与否、有无胎膜早破、分娩方式将其分为早产临产、未足月胎膜早破（PPROM）、足月临产、足月胎膜早破（TPROM）组，以足月择期剖宫产产妇为对照组，每组各20例，分娩后采集其胎膜组织，用免疫组化链霉菌抗生物素蛋白一过氧化物酶连接（SP）法检测Ets-1蛋白的表达水平及定位，每组选取6例以逆转录（RT）-PCR技术检测Ets-1mRNA的表达水平，并进行半定量分析。结果（1）各组胎膜组织中Ets-1mRNA的表达：早产临产、PPROM、足月临产、TPROM、对照组胎膜组织中Ets-1mRNA表达水平分别为0．342±0．016、0．6034-0．027、0．325±0．013、0．5824-0．075、0．139±0．012．PPROM组和TPROM组均高于对照组，分别比较，差异均有统计学意义（P〈0．05）；早产临产组与足月临产组，PPROM组与TPROM组比较，差异均无统计学意义（P〉0．05）。（2）Ets-1蛋白在胎膜组织的定位：Ets-1蛋白集中在羊膜和绒毛膜的间质层、滋养层细胞的胞质和胞核中表达；细胞内可见清晰的棕黄色颗粒。在羊膜上皮细胞中未见Ets-1蛋白表达。（3）各组胎膜组织中Ets-1蛋白的表达：早产临产、PPROM、足月临产、TPROM、对照组胎膜组织中Ets-1蛋白表达水平分别为0．552±0．018、2．853±0．174、0．538±0．042、2．731±0．090、0．214±0．013，PPROM组与TPROM组均高于对照组，分别比较，差异均有统计学意义（P〈0．05）；但早产临产组与足月临产组，PPROM组与TPROM组胎膜组织分别比较，差异均无统计学意义（P〉0．05）。结论Ets-1在人类胎膜组织中有表达，且在胎膜早破时表达水平升高。

英文摘要：

Objective To investigate the role of transcription factor Ets-1 involved in premature rupture of membranes （PROM） by detecting its expression and location in human fetal membranes. Methods Between Feb. and Nov. 2007, 100 pregnant women who delivered in the First Affiliated Hospital, Chongqing Medical University were enrolled in this study. According to gestational weeks, rupture of amnioehorionic membranes and delivery mode, those women were classified into preterm labor group, preterm premature rupture of membranes （PPROM） group, term labor group and term premature rupture of membranes （TPROM） group, matched with elective term cesarean sections women as control group. There were 20 pregnant women in every group. The expression and distribution of Ets-1 protein in fetal membranes were detected by immunohistoehemical streptravidin-biotin peroxidase （SP） method and histoseore. In the mean time, 6 cases were chosen from each group randomly and reverse transcription-PCR was used to measure the Ets-1 mRNA expression. Results （ 1 ） The expression of Ets-1 mRNA in fetal membranes were 0. 342±0. 016 in preterm labor group,0. 603 ±0. 027 in PPROM group,0. 325 ＋ 0. 013 in term labor group, 0. 582 ±0. 075 in TPROM group,0. 139 ±0. 012 in control group, respectively. When compared with that in control group, Ets-1 mRNA expression were significantly increased in both PPROM and TPROM group（ P 〈 0. 05 ）. However, it did not show remarkably difference between preterm group and term labor group, as well as between PPROM and TPROM group （ P 〉 0. 05 ）. （ 2 ） Ets-1 was in stromal layers of amniocborionic membranes and in both cytoplasm and nuclei of trophoblast, which were shown with diffuse intracellular positive granularities （brown-yellow） clearly. No expression of Ets-1 was observed in amniotic epithelial ceils. （3） The expression of Ets-1 protein was 0. 552 ± 0. 018 in preterm labor group, 2. 853 ±0. 174 in PPROM group, 0. 538 ±0. 042 in term labor group, 2. 731±0. 090 in TP