中文摘要：

目的：探讨脉冲电磁场（Pulsed Electromagnetic Field,PEMF）对在二维、三维支架材料条件下培养的大鼠骨髓间充质干细胞（Bone Marrow Mesenchymal Stem Cells,BMSCs）活性的影响。方法：采用频率15 Hz、磁感应强度1 m T的PEMF作用于BMSCs,对于二维培养的BMSCs检测增殖活性和碱性磷酸酶（ALP）活性;对于三维培养的BMSCs检测细胞活性、细胞骨架以及增殖率。结果：PEMF处理4 d、7 d可以显著地增加BMSCs的增殖能力（P〈0.05）,PEMF处理14d显著提高了ALP活性（P〈0.05）。PEMF处理不仅能促进接种于支架材料中BMSCs活性和保持其长梭形形态,而且还能显著增加BMSCs在支架材料中的增殖率（P〈0.01）。结论：PEMF在15 Hz、1 m T条件下可以显著地促进BMSCs的增殖和成骨分化,而且能够促进BMSCs在骨支架材料中的活性。

英文摘要：

Objective To investigate the effect of pulsed electromagnetic fields （PEMF） on viability of rat bone marrow mesenchymal stem cells （BMSCs） cultured in two-dimensional （2D） and three dimensional （3D） scaffolds. Methods PEMF of 15 Hz and 1 mT was applied to stimulate BMSCs. The proliferation activity and alkaline phosphatase （ALP） activity of BMSCs cultured in 2D scaffolds and the cell viability, morphology and proliferation rate of BMSCs cultured in 3D scaffolds were examined. Results The proliferation ability of BMSCs was promoted after the PEMF simulation for 4 d, 7 d （P〈0.05） and ALP activity was also significantly improved after the PEMF simulation for 14 d （P〈0.05）. PEMF stimulation promoted the cell viability of BMSCs cultured in scaffolds, maintaining the long spindle shape, and significantly increased the proliferation rate of BMSCs in scaffolds （P〈0.01）. Conclusion PEMF of 15 Hz and 1 mT significantly increases the proliferation and osteogenic differentiation of BMSCs, and promotes the cell viability of BMSCs in scaffolds.