中文摘要：

针对当前农药检测设备所存在的造价高、自动化程度低、试剂耗材量大等问题,提出一种基于纸基微流控芯片的农药光电检测方法。设计一种简单、便携、廉价的纸基微流控芯片。构造桥式复合结构提升微流控酶抑制显色反应的均匀度,设计适用于光吸收反射检测的光路及电路结构,最终创建集化学反应、光吸收反射效应及环境参数控制于一体的便携化农药检测系统试验平台,并对试验平台最佳工艺参数进行测定与优化。试验结果表明：在达到国家检测标准的前提下,所述检测法分辨率可达0.002 mg/L,高于农药速测卡法;在农药检测浓度范围内,所述方法与yps-1 168型便携式农药检测仪检出限相当,但试剂消耗价格降低了94.79%,且检测时间缩短23%。因此设计的纸基微流控芯片农药光电检测系统,为农残检测的便携化研制发展奠定理论基础。

英文摘要：

A photoelectric detection method based on paper-based microfluidic chip in pesticide residues is proposedbecause the existing equipment has the problems of high cost, low degree of automation and large amount of reagentconsumables. We design a kind of simple, portable, inexpensive paper-based microfluidic chip, whose bridge compositestructure can enhance the uniformity of the color reaction of microfluidic enzyme, and also design the light path and circuitstructure suitable for optical absorption reflection detection. The signal amplifying circuit and filter circuit are built toeliminate the interference of clutter and noise in photoelectric detection. Finally, a portable detection system of pesticideresidue is established, which is an integrated test platform of the chemical reaction, the reflection effect in light absorption andthe control of environment parameters. We observe and optimize the light source, chip placement angle, temperature and otherprocess parameters of test platform in Jiangsu University in 2016. The reflection absorption effect of red LEDs （light-emittingdiodes） is tested to be the best among the LEDs with different colors through detecting the paper-based chip color area. Theresponse effect of the system is better when the angle is 10% according to the chip placement angle and reflected voltage data ofphotoelectric detection during the reaction. It is concluded that light absorption is the most under 35℃ and the chemicalreaction is the most sufficient via comparing the color effect of the biochemical reaction of enzyme inhibition under differenttemperatures. We design different concentrations of pesticides, which are used for biochemical reactions of enzyme ：inhibitionin different paper-based microfluidic chips. The resulting product displays blue color region in the paper-based chip, which canbe characterized by the color of the different concentrations of pesticides. We use the photoelectric detection device to detectthe color zone of the chip; the reflected voltage data can be ob