中文摘要：

[目的]获得高效表达并且抗原性较好的VirB5蛋白，并分析其生物信息学功能。[方法]以羊种布鲁氏菌16M基因组为模板扩增VirB5基因，连接表达载体pET-32a，并转化至大肠杆菌（DE3）中表达，用SDS—PAGE和Western Blot检测其免疫学特性，采用信息生物学方法对VirB5基因编码蛋白的理化性质、结构、保守结构域进行分析。[结果]成功克隆出VirB5基因，并在大肠杆菌中成功表达，SDS—PAGE和Western Blot证实表达重组蛋白对布鲁氏菌阳性血清具有反应原性，生物信息学方法推测VirB5基因可能在胞内运输功能方面发挥重要作用。[结论]成功克隆出717bp大小的VirB5基因，并在大肠杆菌中成功表达45kDa大小的蛋白，具有良好的抗原性，且证明VirB5蛋白与布鲁氏菌的胞内运输相关。

英文摘要：

[ Objective] To get high expression and better antigenic protein VirB5, And to analyze its bioinformatics function. [ Methods] Brucella melitensis 16 M genome as a template to amplify VirB5 gene, connected the pET- 32a expression vector, and transformed into E. coli（ DE3）, detection of immunological properties by SDS - PAGE and Western Blot. VirB5 protein - coding genes were analyzed by biological information methods on the use of physical and chemical properties, structure, and conserved domain. [ Results ] VirB5 gene was successfully cloned and highly expressed in E. coli, SDS - PAGE and Western Blot confirmed the expression of recombinant proteins of Brucella positive serum has reactogenicity, VirB5 protein might play an important role in biology and molecular function. [ Conclusion] The 45 kDa VirB5 protein was successful expression in E. coli, which has good antigenicity and the VirB5 protein was associated with the intracellular transport of Brucella.