中文摘要：

将电纺碳纳米纤维（CNF）掺杂于吸附有三联吡啶钌[Ru（bpy）3^2＋]的Nafion聚合物膜中,制成固态电化学发光（ECL）传感器,并将其用于对阿托品的检测。实验表明,CNF的加入能够增强Ru（bpy）3^2＋/阿托品体系的电化学和ECL信号,且Ru（bpy）3^2＋在膜中的电化学反应受扩散控制。在最佳实验条件下,ECL强度与阿托品浓度在1×10^-7～1×10^-4 mol/L范围内呈良好的线性关系,其线性回归方程为logI=6.7408＋0.8148logC（n=8）,r=0.9967;检出限为1×10^-7 mol/L（S/N=3）。考察了此传感器的重现性,对1×10^-5mol/L阿托品重复检测7次,ECL强度的RSD为2.86%。此传感器的稳定性结果令人满意。将此方法用于尿样中阿托品的检测,回收率在81%～88%之间。

英文摘要：

A novel electrochemiluminescence（ECL）sensor based on tris（2,2′-bipyridyl）ruthenium（Ⅱ） （Ru（bpy）32＋）/electrospun carbon nanofiber（CNF）/Nafion composite films was demonstrated for the determination of atropine.The voltammetric and ECL behaviors of the presented sensor were investi-gated.The results indicated that the addition of CNF in the composite films could increase the current and ECL intensity of Ru（bpy）3^2＋,and the immobilized Ru（bpy）3^2＋performed a diffusion-controlled process.Under the optimal experimental conditions,the proposed ECL sensor gave a wide linear range（r= 0.9967）from 1×10^-7 mol/L to 1×10^-4 mol/L with a detection limit（S/N=3）of 1×10^-7 mol/L for atropine.The relative standard deviation for 1×10^-5 mol/L atropine is 2.9%（n=7）,and the present ECL sensor displays outstanding stability.The ECL sensor was also demonstrated for the determina-tion of atropine in human urine sample and satisfactory results were obtained.