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中文摘要：
背景：近年来，静电纺丝法已被认为是一种制备纳米至亚微米级纤维组织工程支架的简便方法。目的：对3种由静电纺丝法制备的纤维膜进行生物学评价。设计、时间及地点：观察性实验，于2009-01／04在杭州师范大学临床医学院完成。材料：电纺丝素蛋白，聚己内酯超细纤维膜（SF70／PCL30，SF50／PCL50）以及丝素蛋白／聚己内酯／纳米羟基磷灰石超细纤维膜（SF50／PCL50-nHA，其中纳米羟基磷灰石含龟为30％）由浙江理工大学省部共建“先进纺织材料与制备技术教育部重点实验室”制备。方法：将L929细胞以5．5×10^8L^-1浓度接种在3种电纺超细纤维膜上进行培养。在1，3，5h和1，4，7d时用MTT法测定细胞黏附和增殖情况，在1d和7d时扫描电镜观察细胞的形态。主要观察指标：L929细胞在电纺SF70／PCL30，SF50／PCL50，SF50／PCL50-nHA超细纤维膜上的黏附和增殖情况；L929细胞的形态特征。结果：相比于没有加入纳米弪基磷灰石的电纺纤维膜，电纺SF50／PCL50-nHA超细纤维膜会较好地提高细胞在其上的黏附和增殖能力。扫描电镜观察也表明，L929细胞可以在电纺SF50／PCL50-nHA超细纤维膜上很好地呈梭形生长，并且在其表面可以观察到丰富的绒毛和伪足，伪足与材料紧密相联。结论：3种电纺超细纤维膜特别是电纺SF50／PCL50-nHA超细纤维膜可以很好地应用于组织再生。
英文摘要：
BACKGROUND： Recent years, electrospining has been widely recognized as a unique and facile technique for producing nanofibers or sub-micron fibers tissue engineering scaffolds. OBJECTIVE： To evaluate the biological property of the electrospun SF70/PCL30, SF50/PCL50 and SF50/PCL50-nHA ultrafine fiber membranes. DESIGN, TIME AND SETTING： The observational experiment was performed at the Clinical College of Hangzhou Normal University from January to April 2009. MATERIALS： Electrospun SF70/PCL30, SF50/PCL50 and SF50/PCL50-nHA ultrafine fiber membranes fabricated by Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, Zhejieng Sci-Tech University. METHODS： The L929 cells were seeded （seeding density： 5.5× 10^5 cells/mL） on electrospun SF70/PCL30, SF50/PCL50 and SF50/PCL50-nHA ultrafine fiber membranes and TCP （Tissue Culture Plate-Control） on 96 well plates and incubated in vitro. The MIT was performed at hours 1, 3, 5, and days 1, 4 end 7. Scanning electron microscopy was performed at days 1 and 7. MAIN OUTCOME MEASURES： Adhesion and proliferation of mice fibroblasts （L929） on electrospun SF70/PCL30, SF50/PCL50 and SF50/PCL50-nHA ultrafine fiber membranes were studied, end morphologic characteristics of cells were observed. RESULTS： The adhesion rate and proliferation ability of cells could be obvious increased on electrospun SF50/PCL50-nHA ultrafine fiber membrane compared to other ultrafine fiber membrane. Meantime, the scanning electron microscopy showed that the L929 cells spread actively on the electrospun SF50/PCL50-nHA ultrafine fiber membrane with fusiform shape, and cells were attached to the surfaces by discrete filopodia and exhibited numerous microvilli on their surfaces. CONCLUSION： The three electrospun membranes, especially the electrospun SF50/PCL50-nHA ultrafine fiber membrane, can be used in tissue regeneration.
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