中文摘要：

为了探寻OmpR和PhoP之间的交叉调节关系，构建了ompR-phoP双缺陷变异株，运用基因芯片分析技术比较了伤寒沙门菌ompR缺陷株、phoP缺陷株及ompR—phoP双缺陷株之间基因表达谱的差异．结果显示OmpR与PhoP共同激活11个基因的转录，主要为外膜孔蛋白相关基因、脂多糖修饰相关基因等，两者在调节特定的膜蛋白、孔蛋白及表面结构成分的表达水平方面发挥着协同作用，OmpR与PhoP对未知功能蛋白基因t0563、t0564也具有协同激活效应，值得进一步关注．OmpR与PhoP共同抑制nanT的转录，阻止唾液酸的转运．另外，ompR缺陷株和phoP缺陷株中未见变化，而ompR-phoP双缺陷变异株中差异表达基因也可能是OmpR和PhoP的共同调节元，但需要进一步验证．

英文摘要：

To explore the cross-regulation network of OmpR and PhoP, ompR-phoP deletion mutant strain was constructed, and S. Typhi genomic DNA mieroarray was used to compare the global transcriptional difference between ompR mutant strain, phoP mutant strain, ompR-phoP mutant strain, and the wild-type strain upon up-shift high osmotic stress. The results showed that transcription of 11 genes were activated and 1 gene was inhibited simultaneously by OmpR and PhoP, respectively. These activated genes encoded outer membrane proteins and lipopolysaccharide modification proteins, which indicated that OmpR and PhoP can together promote the expression of membrane protein, porin and structrual component of bacterial surface. Furthermore, among these activated genes, t0563 and t0564 with unknown functions are worth further research. On the other hand, OmpR and PhoP inhibited the transcription of nanT simultaneously to prevent the transport of sialic acid. Genes with transcriptional difference only observed in ompR-phoP mutant strain may also be the regulon of OmpR and PhoP.