中文摘要：

为给小麦抗穗发芽育种提供基础材料和参考依据，以杨麦5号为受体，对转反义Trxs基因小麦00TY5的后代株系进行PCR分子检测，并对阳性株系进行了穗发芽和籽粒发芽试验。结果表明，PCR检测T4代14个转基因株系中阳性株系占78．5％．具有抗穗发芽特性的株系占阳性株系的54．5％。这些抗性株系在成熟前5d至成熟后10d．各株系与扬麦5号相比平均穗发芽时间推迟2．1d，穗粒发芽率和穗发芽度分别降低26．4％和52．8％；籽粒发芽开始时间延迟2d．籽粒发芽率和发芽指数分别降低40．8％和43．7％，差异达到显著或极显著水平。成熟后25d，各株系与扬麦5号的各种发芽参数差异减少或差异不明显。相关分析表明，穗发芽参数与籽粒发芽参数呈极显著相关关系。

英文摘要：

The transgenic wheat lines 00TY5 with anti-trxs gene had been obtained by transforming Yangmai 5. The PCR screenins on these lines, pre-harvest sprouting and seed germination experiments were carried out for the positive lines. The results indicated that the posilive lines with the target gene were 78.5% among the transgenic lines by PCR screening. The frequenly of frequency of the resistant sprouting and germination lines as 54.5% among the 11 positive transgenic ones. Compared with Yangmai 5, from 5 days before maturity to 10 days after maturity, the positive lines showed stronger resistance to spikes sprouting, and the average sprouting date was postponed by 2. 1 days, the spike-seed germination ratio and the spike sprouting degree was reduced by 26.4% and 52.8%, respectively. The date of seed germination was postponed by 2.0 days, and seed germination ratio and germination index were reduced by 40. 8% and 43.7%, respectively, which indicated a significant difference existed between these positive lines and the receipter variety. But 25 days after maturity, the germinating capacity of these positive lines recovered gradually, and no significant differences were found between them. Correlation analysis indicated that significant positive correlations existed between the spike sprouting parameters and the seed germinating parameters.