中文摘要：

目的：确定NALP3在淋球菌诱导的人宫颈癌细胞（Hela）促炎细胞因子产生中的作用及其激活途径。方法：将Hela细胞分为空白对照组、Bayll-7082抑制剂组、淋球菌组和淋球菌＋Bayll-7082抑制剂组。荧光定量PCR检测Hela细胞内NALP3mRNA表达，WesternBlot检测Hela细胞核内NF-κBp65和胞质内caspase-1p20蛋白质水平，酶联免疫吸附法（ELISA）测定各组细胞培养上清液中ITJ-1β的含量。结果：淋球菌组细胞内NAIU3mRNA表达是空白对照组的5．8倍，核内NF-κBp65和胞质内caspase-1p20蛋白质水平是空白对照组的3．2倍和3．1倍，细胞培养上清液中的IL-1G含量显著高于对照组（P〈0．01）。结论：淋球菌通过活化NF-κＢ诱导Hela细胞NALP3的表达并活化caspase-1和IL-1β。

英文摘要：

Objective： To determine the role of NALP3 in the production of pro - inflammatory cytokines induced by Neisseria gonorrhoeae （Ng） in Hela ceils and the activation pathway of NALP3. Methods： Hela cells were ran- domly divided into four groups： control group, Bayl 1 - 7082 group, Ng group and Ng ＋ Bayl 1 - 708 group. The mR- NA expression of NALP3 in the cells was determined by real - time PCR. Western Blot was preformed to detect the protein expression of NF - κB p65 in nucleus and caspase - 1p20 in cytoplasm. The protein of interleukin - 1β were determined by enzyme - linked immunosorbent assay. Results： Compared with control group, the rnRNA expression of NALP3 was 5.8 fold （ P 〈 0.01 ）, the protein expression of NF - feb p65 in nucleus and easpase - 1p20 in cyto- plasm were 3.2 fold and 3.1 fold, respectively. The concentration of IL- 1β in supernatant was much higher in cul- tural groups than in controls （P 〈 0.01）. Conclusion： Ng induces the expression of NALP3 in Hela cells through activating transcription factor NF- κB, and activate caspase- 1 and pro- inflammatory cytokines IL- 1β.