中文摘要：

对辣椒细胞质雄性不育系‘9704A＇和其同核保持系‘9704B’早期花蕾转录组的Solexa测序结果进行分析，发现1个差异性表达基因，其在不育系中的表达比在保持系中的表达高10倍，该基因与番茄、葡萄、拟南芥中的hsf基因同源性分别为87％、73％和70％，推测该基因为辣椒的而妒基因。根据该基因序列设计引物，从辣椒‘9704A＇中克隆该基因的全长及核心cDNA序列，克隆到的hsf全长与核心序列与转录组测序获得的序列一致，进一步分析表明，该基因属于矗妒基因家族成员。用定量PCR分析矗旷基因在‘9704A＇和‘9704B’2种辣椒的根、茎、叶和晚期花蕾中的表达情况，结果表明，该基因在不育系叶组织的表达量为其在保持系叶组织表达量的13．7倍，在不育系晚期花蕾中的表达量也为其在保持系晚期花蕾的3倍，而在2种辣椒系的茎中的表达量都很低，在2种辣椒系的根中的表达量均较高。

英文摘要：

Transcriptom Solexa sequencing was conducted on pepper early flower buds of the CMS line ＇9704A＇ and its correspondence homonuclear maintainer line ＇9704B＇ previously. A gene that expressed 10 times higher in pepper cytoplasm male sterile （CMS） line ＇9704A＇ than it is in the maintainer line ＇9704B＇ was identified. Gene homology analysis of the cloned hsfgene showed that the gene shares 87% homology with the hsfin Solanum lycopersicum, 73% in Vitis vinifera and 70% in Arabidopsis thaliana. According to the gene sequence we designed the primers and cloned the core sequence and hsfcDNA in pepper ＇9704A＇. Sequence analysis showed that the cloned hsfcDNA was consistent with transcriptom Solexa sequencing. Gene analysis showed that the gene is one of the plants hsfgene family members. The real-time fluorescence quantitative RT-PCR primers were designed and the RT-PCR of the gene was carried out with the compared tissues in ＇9704A＇ and ＇9704B＇, using actin as reference gene. The result showed that the hsfgene expression in ＇9704A＇ leaf is 13 times higher than it is in the maintainer line and in ＇9704A＇ later flower bud is 3 times higher. The gene expressed very low in the stems of the two materials while high in the roots.