中文摘要：

利用响应面设计对超临界CO2抗溶剂法制备负载β-胡萝卜素的玉米蛋白纳米粒的工艺进行研究。分别以β-胡萝卜素的负载量和包埋率为优化指标,系统考察芯材比、温度、压力等主要因素对玉米蛋白包埋β-胡萝卜素的交互影响,采用SEM、DLS和XRD对产品形貌、粒径和结构等进行表征。结果表明：芯材比对载药量和包埋率的影响最显著,其次是压力,最后是温度。当芯材比1：10（质量比）、温度55℃、压力8MPa时,β-胡萝卜素的负载量达到最大值8.73%;当芯材比1：30、温度35℃、压力16MPa时,β-胡萝卜素的包埋率有最高值85.4%。玉米蛋白和β-胡萝卜素形成Matrix结构,颗粒平均粒径大约150nm,粒径分布窄,球形度好。

英文摘要：

Response surface methodology was used to optimize the preparation ofβ-carotene-loaded zein nanospheres by supercritical anti-solvent technique.The effects of the ratio betweenβ-carotene and zein,temperature,and pressure as well as interactions among them onβ-carotene loading and embedding rate were investigated,and the morphology,particle size and structure ofβ-carotene-loaded zein nanospheres were characterized by SEM,DLS and XRD.The results indicated that core/wall ratio had the most significant effect onβ-carotene loading and embedding rate,sequentially followed by pressure and temperature. β-carotene loading reached its maximum level of 8.73%,when theβ-carotene/zein ratio was 1：10,the temperature 55℃,and the pressure 8 MPa.β-carotene showed a maximum embedded rate of 85.4%under the following conditions：β-carotene/zein ratio of 1：30,temperature of 35℃,and pressure of 16 MPa.The structure ofβ-carotene-loaded zein particles was a matrix,the average particle size was about 150 nm with a narrow distribution,and the spherical degree was high.