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中文摘要：
用吸收装置改变荧光分光光度计入射光的光路。获得光源的强度光谱和样品的透光光谱，并在同一台仪器上建立一种简单的分离表观吸收光谱中吸收和散射成分的方法．通过对TAPP（α，β，γ，δ-四-（对三甲氨基苯基）卟啉）与DNA作用的共振光散射光谱进行校正，结果表明。校正表观分子吸收后，灵敏度提高了2倍左右．校正仪器影响后，从F-2500和F-4500荧光分光光度计获得表观吸收光谱中分离出的TAPP与DNA作用的散射成分，其形状基本一致．
英文摘要：
By changing the propagation direction of the incident light beam of a common spectrofluorometer through an absorption cell holder, the incident light spectrum and the transmitted light spectrum are directly measured through a spectrofluorometer. A simple corrected resonance light-scattering technique was established to separate absorbance and scattering components from apparent absorption spectrum. With measurement of the resonance light scattering signals, after which the molecular effect was corrected, the interaction of α,β,γ,δ-Tetrakis[4-（trimethylammoniumyl） phenyl] porphine （TAPP） with DNA, the detection sensitivity could be improved about two fold and the shape of scattering components separated from FL2500 and F-4500 spectrofluorometer was identical when the instrumental effect was corrected.
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