中文摘要：

目的：观察低氧向心运动后Desmin的变化,比较不同恢复方式对Desmin的影响,从基因表达角度探讨Desmin的调节机制。方法：雄性SD大鼠48只,随机分为8组：对照组（C）、低氧运动后即刻组（HE）、低氧运动后常氧恢复1、27、d组（N1d、N2d、N7d）,低氧运动后低氧恢复1、2、7 d组（H1d、H2d、H7d）。采取递增负荷进行低氧下10°跑台运动,低氧环境的氧浓度为12.7%。采取Western Blotting和RT-PCR分别检测大鼠腓肠肌Desmin的蛋白和基因表达水平。结果：低氧运动后即刻Desmin并没有丢失;低氧恢复和常氧恢复各组之间Desmin含量差异均无显著性意义。与对照组比较,运动后即刻Desmin基因表达有增加的趋势;常氧恢复和低氧恢复期12、d组均高于对照（P〈0.05）。但低氧恢复基因表达与常氧之间差异显著（P〈0.05）。结论：低氧向心运动没有使Desmin含量出现丢失或下降现象。基因调节在小分子量骨架蛋白Desmin的代谢中起重要作用。低氧使蛋白降解加速,同时启动基因表达途径进行蛋白合成。

英文摘要：

The purpose of this study was to analyze the influence of hypoxia on Desmin in rats skeletal muscle after hypoxic exercise, compare the difference between hypoxic and normoxic recovery, and analysis the mechanism of regulating Desmin by the gene expression of skeleton protein. Fourty eight Male SD rats were randomly divided into eight groups： control group（ C： n = 6）, 0 h after hypoxia exercise group（ HE： n = 6）, 1、2 、 7d groups in normoxia （Nla： n = 6; N2a： n = 6; N7a： n = 6） ,1、2、7d groups in hypoxia（ Hid： n = 6; H2d： n = 6; H7a： n = 6）after hypoxia exercise of progressive loading in treadmill （10°）. The concentration of oxygen was 12.7 %. The tissues collected from gastrocnemius muscle of the rats. The protein content and gene expression of Desmin was detected by Western Blotting and RT - PCR. The results showed that the content of Desmin didn＇t losed at Oh after hypoxic exercise. There were no significant difference between the content of Desmin during hypoxic recovery and normoxic recov- ery. There was an increased trend of gene express of Desmin at 0 h after hypoxic exercise. Compare to control group, the gene express was significant higher in 1 d and 2d of normoxic recovery and hypoxic recovery（ P 〈 0.05）. But there was significant difference between the gene express of hypoxie recovery and normoxic recovery. The re- suits showed that the Desmin was not losed in concentric exercise under hypoxia. The regulation of gene was the one of main causes of the rapidly synthesis of Desmin. The content of Deasmin maybe promoted the degradation of protein by hypoxia, then caused the protein synthesis by expression of gene