本研究首次利用诱导性多能干细胞(iPS细胞)通过四倍体囊胚注射得到存活并具有繁殖能力的小鼠。研究中,我们制备了37株iPS细胞系,其中3株iPS细胞系获得了共计27个活体小鼠,经多种分子生物学技术鉴定,证实该小鼠确实从iPS细胞发育而成。其中一些小鼠现已发育成熟并且繁殖了后代,这是世界上第一次获得完全由iPS细胞制备的活体小鼠,有力地证明了iPS细胞具有真正的全能性。这项工作为进一步研究iPS技术在干细胞、发育生物学和再生医学领域的应用提供了技术平台,将iPS细胞研究推到了新的高度,也为中国在这一国际热点研究领域取得领先地位做出了重要的贡献。
We used viral vectors to introduce four genes into mouse fibroblast cells to reprogram these somatic cells into pluripotent cell in order to create iPS cells.After carrying out a standard set of tests to check whether the reprogramming had worked,our research group confirmed that we have generated 37 iPS cell lines.Then we tested the pluripotency of these iPS cell lines by tetraploid complementation and 6 of 37 iPS cell lines generated 27 live mice.12 mice that were mated produced healthy offspring and for now,they have got hundreds of second generation,and more than 100 third-generation mice which were healthy and viable.