为了揭示不同树龄银杏的根、茎、叶解剖结构以及内生菌分布情况,本研究采用石蜡切片法对银杏(Ginkgo biloba L.)根、茎、叶显微解剖结构进行了观察。结果显示:(1)一年生银杏幼根不含树脂道,内生菌含量低,而皮层中含有大量蛋白细胞;多年生银杏老根含有较多树脂道,皮层细胞中含有大量内生菌并有针晶物质分布,未发现蛋白细胞。(2)一年生银杏幼茎有明显的角质层,皮层分布有大量蛋白细胞,韧皮射线及髓部发达,其中髓由大量薄壁细胞构成并且有蛋白细胞分布,未观察到树脂道但有簇晶物质存在。(3)多年生银杏叶片海绵组织疏松,具有树脂道,叶肉细胞含有簇晶物质;气孔下陷并具有耐旱的结构特点。本结果可为研究不同树龄银杏对环境的适应性变化以及内生菌特点提供参考。
To confirm the anatomical structure and distribution of endophytes in the vegetative organs of different aged ginkgoes,we observed the anatomical structure of Ginkgo biloba L.roots,stems and leaves by paraffin section. Results showed that:( 1) The root of annual G.biloba did not have a resin canal,but did have a few endophytes and a large number of albuminous cells in the cortex cells. The perennial root had a resin canal and many endophytes,as well as needle-like crystals,but did not have albuminous cells.( 2) The young stem had an obvious cuticle,numerous albuminous cells in the cortex cells,and a developed phloem ray and central pith. The latter consisted of a large number of parenchyma cells,with albuminous cells and crystal druses but without a resin canal in the pith.( 3) The spongy tissue of G. biloba leaves had a loose structure. There were resin canals and crystal druses in the mesophyll cells,and stomata with invagination under the epidermis,indicating that G.biloba had anatomical characteristics for drought tolerance. Based on the structure of the vegetative organs,this study helps clarify the relationship between different aged G. biloba and the ecological environment and provides reference for the further study of G. biloba endophytes.