中文摘要：

目的观察中药单体绿原酸对深静脉血栓形成(DVT)大鼠Th17/Treg亚群失衡的影响。方法将60只大鼠随机分为6组各10只,A、B、C、D组Reyers法建立大鼠DVT模型,分别给予绿原酸40、20、10 mg/kg及等量生理盐水灌胃7 d;E组为假手术组,F组不处理。分离大鼠脾脏单核细胞,流式细胞术检测各组Th17、Treg亚群比例,ELISA法检测大鼠血清IL-17、TGF-β蛋白,分析Th17/Treg比例与血清IL-17、TGF-β相关性。结果与E、F组比较,B~D组脾脏Th17亚群比例升高、Treg亚群比例降低、Th17/Treg升高、IL-17表达升高、TGF-β表达降低,P均<0.05;与D组比较,A、B、C组Th17亚群比例降低、Treg亚群比例升高、Th17/Treg降低、IL-17表达降低、TGF-β表达升高(P均<0.05);Th17/Treg值与IL-17呈正相关(r=0.618,P<0.05),与TGF-β呈负相关(r=-0.577,P<0.05)。结论在大鼠DVT发病过程中存在Th17/Treg亚群失衡,中药单体绿原酸诱导T细胞向Treg亚群偏移,逆转Th17/Treg亚群失衡状态,进而减轻炎症反应,保护血管内皮,抑制血栓形成。

英文摘要：

Objective To evaluate the role of Th17/Treg shift in deep vein thrombasis ( DVT) rats and elucidate the mechanism of chlorogenic acid to treat DVT. Methods 60 rats were divided randomly into six groups (10 rats in each group). A, B, C, D groups according to the Reyers method to establish the model of DVT, give chlorogenic acid respec-tively 40, 20, 10 mg/kg and the amount of normal saline lavage in succession for 7 days.10 rats with sham operation were taken as E group.F group no processing. The proportions of Th17 subset and Treg subset were detected by flow cytometry. The protein expressions of IL-17, TGF-βin serum were analyzed by ELISA assay, and to analyze the correlation of the pro-portion of Th17/Treg with serum levels of inflammatory markers. Results Compared with E and F groups, the proportion of Th17 subset increased while that of Treg subset decreased, the protein expression of IL-17 enhanced while that of TGF-βinhibited markedly in B-D groups (all P<0.05).Compared with D group, the proportion of Th17 subset was down-reg-ulated while that of Treg subset was up-regulated significantly A-C groups (all P<0.05), the protein expression of IL-17 decreased while that of TGF-βincreased obviously in A-C groups (all P<0.05).The values of Th17/Treg and IL-17 were positively correlated, and negatively correlated with TGF-β, all P<0.05.Conclusions Th17/Treg shift was involved in DVT.Chlorogenic acid down-regulated the Th17 subsets and up-regulated the Treg subsets;thereby, chlorogenic acid exer-ted the curative effect on DVT rats.