中文摘要：

目的研究补骨脂素（psoralen,PSO）对体外培养的小鼠成骨细胞（OB）分化及核因子-κB受体激活配体（receptor activator of nuclearfactor-κB ligand,RANKL）和骨保护素（osteoprotegerin,OPG）表达的影响。方法取第1代BALB/c小鼠颅盖骨成骨细胞,将PSO以0.1、1、10μmol/L3种浓度分别加入新生大鼠颅骨成骨细胞培养液中,MTT法观察各组药物对成骨细胞的增殖作用并绘制细胞生长曲线;用PNPP法测定成骨细胞内碱性磷酸酶（alkaline phosphatase,ALP）活性;RT-PCR法检测成骨细胞OPG和RANKL的转录水平。结果细胞生长曲线显示各组成骨细胞数量均随时间延长而增加,中、高浓度PSO能显著提高成骨细胞的ALP活性,促进OPG、RANKL的表达（P〈0.05）,OPG/RANKL升高（P〈0.05）。结论低浓度PSO（0.1μmol/L）对骨更新作用不明显,而中、高浓度PSO（1、10μmol/L）能通过上调OPG、RANKL mRNA表达及OPG/RANKL比例,促进成骨细胞的生成功能,增强骨更新。

英文摘要：

Objective To investigate the role of psoralen on the differentiation of murine osteoblasts （OB） , and to observe the expressions of receptor activator of nuclear factor-κB ligand（RANKL） and osteoprotegerin（OPG） in vitro. Methods The calvarial OB of newborn BALB/c mice were cultured in MEM medium containing 10 % NCS. Psoralen was supplemented into the culture medium of osteoblast at 0. 1,1 and 10 μmol/L respectively. MTT was used to observe the proliferation and the growth curve was obtained. The osteoblasts alkaline phosphatase（ALP） activity assays were performed by PNPP method , and mRNA expressions of OPG and RANKL were determined by RT- PCR. Results The growth curve showed that the OB quantities of each group had increased accompanied with time. Higher and middle concentration of psoralen significantly increased the ALP activity in OB in vitro, up - regulated the mRNA expressions of RANKL and OPG, increased the ratio of OPG/ RANKL. Conclusions Lower dosage of psoralen （0. 1 μmol/L） has no significant effect on bone turnover, but higher and middle dosage of psoralen （ 1,10 μmol/L） can enhance bone turnover through facilitating the formation and activity of OB via up- regulating the expressions of RANKL and OPG and increasing the ratio of OPG/RANKL.