A method for determining Mg/Ca of stalagmite by LA-MC-ICPMS is studied. 24Mg/44Ca (Mass 22, intensity of 44Ca2+ , collected by ion counting and mass 24, intensity of 24Mg+, collected by Faraday cup) ratios were measured in replace of Mg/Ca ratios. Both diameter of laser sampling spot and laser moving increment were 20 μm. The curve of Mg/Ca vs. distance directly was obtained. The result indicates that relative Mg/Ca ratios changed from 0.5 to 2.0 in this stalagmite and it is enough to reflect environmental factors act.