中文摘要：

目的 研究在三维细胞培养条件下前列腺细胞（RWPE-1）腺泡样结构的形成及电离辐射的影响.方法 采用三维培养RWPE-1细胞,Western blot检测自噬相关基因Beclin 1及p53、p21的变化,制备Beclin 1 RNAi表达载体并感染细胞,免疫荧光染色和共聚焦法检测电离辐射作用后细胞形态学变化.结果 三维培养条件下RWPE-1细胞聚积形成腺泡样结构（Acini）,Acini组成细胞出现明显极化,2和5 Gy照射后,Acini结构破坏,少量细胞发生死亡;经Beclin 1基因沉默后,对照组细胞仍呈聚积趋势,2和5 Gy照射后,Acini结构不再形成,细胞发生广泛性死亡.结论 电离辐射抑制RWPE-1细胞Acini结构形成,Beclin 1基因可能在其中发挥一定作用.

英文摘要：

Objective To observe the formation of acini structure of prostate cells under 3-dimensional （3-D） condition and the effects of radiation on it. Methods RWPE-1 cells were cultured under 3-D condition, Western blot was used to detect the expressions of Beclin 1, p53 and p21, in Beclin 1 knock-down cell model, i. e. ,RWPE-Bi, immunofluorescence staining and confocal microscopy were used to detect the morphological characteristics of RWPE cells irradiated by 60^Co. Results In 3-D culture,acini was observed,characterized by sphroid structure and cell polarity. After radiation, the acini was destructed and cell death was found, especially in RWPE-Bi compared with RWPE-1. Conclusions Radiation could suppress the formation of acini structure of prostate cells and Beclin 1 might play a role in this process.