中文摘要：

目的：探讨RNA干扰技术沉默Survivin基因对鼻咽癌细胞CNE-2恶性表型的影响。方法：设计、合成针对Survivin基因的干扰序列，并将干扰序列构建至pGCsVU6／GFP载体，稳定转染CNE-2细胞；逆转录聚合酶链反应（RT-PCR）、Western blot鉴定干扰效果；经四甲基偶氮唑蓝（MTT）法、流式细胞术、平板克隆形成实验分析沉默Survivin基因后，CNE-2细胞生长速度、细胞周期及恶性表型的改变。结果：沉默Survivin基因后，CNE-2细胞Sur-vivin mRNA、蛋白质表达水平均明显下调；细胞生长速度下降，G1期细胞增多，S期细胞减少，克隆形成能力明显减弱。结论：Survivin基因在CNE-2细胞生长、细胞周期及恶性表型等方面都起着重要的作用。

英文摘要：

Aim：To investigate the effects of RNAi-mediated gene silence of Survivin on the prolifera-tion and malignant phenotype of nasopharyngeal carcinoma cells. Methods： Nasopharyngeal carcinomacell line CNE-2 was transfected by Survivin small interfering RNA （siRNA） and the efficacy of Survivinsilencing was determined with RT-PCR and Western blot. The growth and the malignant phenotype ofCNE-2 was examined by tetrazolium blue （MTI？） assay, flow cytometry and colony formation assay. Re-suits： The level of Survivin gene in CNE-2 was obviously declined after transfected with survivin siRNA.The cell growth rate and clone forming ability was significantly decreased. Morever, the distribution ofcell cycles of CNE-2 cells was changed. Conclusion：Survivin gene plays an important role in prolifera-tion, cell cycle and the malignant phenotype of CNE-2.