丙烯酸甲酯是一种对人体有害的广泛使用的化工原材料。该研究构建了一个以市售陶瓷球为载体的3层的生物滴滤池,并接种了活性污泥,用来处理丙烯酸甲酯废气,并采取PCR-DGGE技术对滴滤池中的细菌群落结构进行了分析。实验结果表明,该研究构建的生物滴滤池系统表现出对丙烯酸甲酯废气的卓越处理性能,当EBRT大于266 s进气浓度小于0.19 g/m3时,在排气口检测不到丙烯酸甲酯废气残留。变形菌门是生物滴滤池中的最优势菌群体,其次是放线菌门和厚壁菌门。Desulfovibrio gigas,Variovorax paradoxus,Dokdonella koreensis,Pseudoxanthomonas suwonensis,Azorhizobium caulinodans,Hyphomicrobium denitrificans,Hyphomicrobium sp.和Comamonas testosteroni共同构成去除丙烯酸甲酯废气的细菌群落。
Methyl acrylate as a widely used raw chemical material is toxic to human. In order to treat waste gas containing methyl acrylate, a bench scale three-layer bio-trickling filter (BTF) packed with ceramic particles and immobilized with activated sludge was set up. The experiment with the BTF exhibited excellent removal efficiency that no methyl acrylate could be detected when EBRT was larger than 266 s and inlet concentration was lower than 0.19 g/m3. Furthermore, technique of PCR (polymerase chain reaction) combined with DGGE was used to detect the bacterial communities at different layers of the BTF, which showed that Phylum Proteobacteria was predominantly represented in the bacterial community, followed by Actinobacteria and Firmicutes. D. gigas, V. paradoxus, D. koreensis, P. suwonensis, A. caulinodans, H. denitrificans, Hyphomicrobium sp. and C. testosterone, and it was the bacterial communities in the BTF that played a vital role in removing methyl acrylate from waste gas.