中文摘要：

根据猪链球菌谷氨酸脱氢酶基因和血清型1型、2型、1/2型、7型、9型和14型的荚膜多糖编码基因核酸序列,分别设计猪链球菌种和血清型特异性引物,建立并优化多重PCR检测方法,检测分析种属背景明确的73株菌株（其中猪链球菌49株、其他对照菌株24株）及临床分离样本94株（包括四川资阳临床分离样本45株）。其中73株种属背景明确菌株多重PCR种检测结果符合率为87.5%,6种主要致病血清型检出率可达100%。24株对照菌株在种和血清型检测均为阴性。对45株四川猪链球菌病暴发现场分离菌株进行检测,其中41株为猪链球菌2型。上述结果提示建立的多重PCR方法对猪链球菌种及主要致病血清型的检测具有较好的特异性和敏感性,可用于猪链球菌病的快速诊断和流行病学调查。

英文摘要：

A multiplex PCR assay for detection and identification of various streptococcus suis strains in tonsillar specimens from pigs was developed and evaluated. In this research five distinct DNA targets were chose： One target, based on the gdh gene, encoded the glutamate dehydrogenase of streptococcus suis, can be applied to the detection of streptococcus suis at species level. The other targets, based on SS capsular polysaccharide （cps） genes specific for serotypes 1（and 14） ,2 （and 1/2）,7,9 were amplified by multiplex PCR. 73 isolates,which included 49 strains of streptococcus suis and 24 strains of negative control,and 94 clinical specimens （45 clinical strains in Sichuan）were detected by the multiplex PCR assay. In 73 streptococcus suis strains, 64 （87.5%） strains were gdh^＋. There were no PCR products in strains of serotypes 13,19,30,32,33,34. The multiplex PCR could detect all strains of serotypes 1 （and 14）, 2 （and 1/2）, 7,9. In 45 clinical strains in Sichuan, the PCR results of 41 strains were gdh^＋cps2^＋. These results indicated that the PCR can be used as a reliable species-specific molecular diagnostic reagent and a serotype-spe- cific method for the detection of streptococcus suis and six main serotypes 1,2,1/2,7,9 and 14, respectively. The PCR method has potential value for clinical and epidemiological applications.