中文摘要：

利用中国臭氧FACE（Free—Air O3 Concentration Enrichment，开放式空气臭氧浓度增高）试验平台，研究了近地层臭氧浓度升高条件下，2009年和2010年的稻田土壤微生物功能多样性的响应规律。结果发现，1．5倍当前臭氧浓度下稻田土壤水溶性有机碳含量从100．0—110．9μgg-1降低至81．3—94．5μgg-1。臭氧浓度升高下BIOLOG平均吸光值（AWCD）有低于对照的趋势。多样性指数结果表明，臭氧浓度升高50％对稻田土壤微生物丰富度、优势度及均一度无显著影响。2010年碳源底物利用的主成分分析显示，1．5倍当前臭氧浓度下稻田土壤微生物对碳源底物的利用方式发生变化。本研究揭示近地层臭氧浓度升高可能对稻田土壤微生物产生积累效应，并通过微生物碳源底物有效性的改变最终影响土壤微生物的功能多样性。

英文摘要：

Effect of elevated surface O3 concentration （ -70 nmol mol-l） on functional diversity of soil microbial communities in paddy field under the rice/wheat rotation system was investigated in 2009 and 2010 through determination of water-soluble organic carbon （WSOC） content and BIOLOG index, by making use of the O3-FACE （ Free-Air ozone Concentration Enrichment） platform of China. Resuhs show that with O3 concentration elevated, both WSOC content, and AWCD （Average Well Colour Development ） tended to decrease. Diversity indexes determined in the two years indicate that elevated O3 concentration did not have much effect on, Shannon, Simpson and McIntosh indices. PCA results in 2010 show that elevation of O3 concentration affected the edaphon＇ s ability of utilizing substrates of some specific carbon sources. The findings of the experiment suggest that under elevated O3 concentration （ - 70 nmol mol-1 ） , functional diversity of soil microbes was significantly increased in rice field.