中文摘要：

以内蒙古地区骆驼盘尾丝虫病流行病学调查为基础，基于细胞色素氧化酶工（COⅠ）基因的分子标记特点，进而为骆驼福斯盘尾丝虫传播媒介的研究奠定基础。在骆驼屠宰季节，进行骆驼福斯盘尾丝虫感染情况的检查，并分离虫体，提取DNA，用PCR方法扩增CO工基因，然后克隆和测序，并运用MEGA5．05和DNAStar7．1软件进行序列分析。结果显示，骆驼福斯盘尾丝虫主要寄生于骆驼的项韧带间隙，骆驼的平均感染率为92．2％，其中阿拉善左旗骆驼感染率高达100％，最大感染强度为每峰骆驼23个结节。对福斯盘尾丝虫COⅠ基因的序列分析结果表明，该基因长689bp，与GenBank中公布的同属线虫其他种的COⅠ基因的同源性介于89．8％～93．8％之间。对内蒙古地区骆驼感染福斯盘尾丝虫的状况进行了调查，并在国内外首次对福斯盘尾丝虫COⅠ基因序列进行了测序分析，为该虫种的分子标记及传播媒介的进一步研究奠定了重要基础。

英文摘要：

Based on the epidemiological investigation of camel onchocerciasis in Inner Mongolia, the ex- periment was conducted to study the charateristics of molecular markers of cytochrome oxidase I （CO I ） gene for verifying the vector of Onchocerca fasciata. Firstly,the camels were killed and carefully examined for the nodules caused by O. fasciata. Subsequently,adult worms were separated and their DNA were ex- tracted. Then the mitoehondrial CO I gene was amplified by PCR using the universal primers,followed by subcloning and sequencing. Lastly, sequence analysis was done by MEGAS. 05 and DNAStar7.1. In result, O. fasciata mainly lived in the interspace of the camel nuchal ligament and formed nodules. The average in- fection rate was 92.2% ,and it was high up to 100% in Alashanzuoqi. Meanwhile,the highest infection in- tensity was 23 nodules per camel. The partial CO l gene sequence was 689 bp in length and the similarity was from 89.8% to 93. 8% compared with different Onchocerca species. The preliminary investigation of O. fasciata was conducted in Inner Mongolia,and it was the first time to report the CO I gene sequence of O. fasciata. The result provided very important information for further study of the vectors and molecular markers of O. fasciata.