中文摘要：

鸡马立克氏病病毒（MDV）经腹腔注射感染8日龄雏鸡,攻毒15 d后检测硫酸化人参总皂苷对鸡外周血白细胞数和淋巴细胞增殖活性的影响,并利用半定量RT-PCR方法分析外周血淋巴细胞IFN-γmRNA的表达水平。结果表明,MDV感染能引起鸡淋巴细胞百分比相对增多（P〈0.01）,人参总皂苷及其硫酸化人参总皂苷不能改变MDV所致变化（P〉0.05）,且硫酸化人参总皂苷与人参总皂苷之间没有差异（P〉0.05）;MDV感染极显著抑制淋巴细胞增殖（P〈0.01）,人参总皂苷能明显改善MDV对感染鸡淋巴细胞增殖的抑制（P〈0.01）,硫酸化人参总皂苷促进了MDV所致的抑制状态（P〈0.01）;人参总皂苷及其硫酸化人参总皂苷均能增强MDV所致鸡淋巴细胞IFN-γmRNA的表达,与健康组相比,硫酸化人参总皂苷极显著增加IFN-γmRNA表达（P〈0.01）。结果提示,硫酸化人参总皂苷对人工感染MDV鸡的淋巴细胞活性具有调节作用,其作用比人参总皂苷的作用更强。

英文摘要：

Eight-day-old chickens were injected peritoneally with Marek＇s disease virus （MDV） to establish MD model, and the effects of sulfated Total Saponin of Panax Ginseng（s-TSPG） on the number of leucocytes, proliferation of lymphocytes, and the expression level of IFN-γ mRNA in peripheral blood lymphocytes by semi quantitative reverse transcription-PCR were evaluated besed on the Model at 15 days postinfection. The results showed that the percentage of lymphocytes in chicken infected by MDV was relatively increased （P〈0.01）, s-TSPG and TSPG did not change the increasing level （P〈0.05）, and there was no difference between s-TSPG and TSPG （P〈0.05）. The lymphocyte proliferation were significantly depressed in chicken infected by MDV （P〈0.01）, and TSPG improved this suppression （P〈0.01）, while s-TSPG promoted this suppression （P〈0.01）. The expression levels of IFN-γ mRNA from lymphocytes induced by MDV were enhanced by s-TSPG and TSPG, and the expression level of IFN-γ mRNA enhanced by s TSPG was significantly higher than that of the control group （P〈0.01）. These data suggested that the regulating effect of s-TSPG on the activity of peripheral lymphocyte of chicken infected by MDV is higher than that of TSPG.